Immunology Laboratory detail syllabus for Biotechnology (Biotech), 2017 regulation is taken from Anna University official website and presented for students of Anna University. The details of the course are: course code (BT8712), Category (PC), Contact Periods/week (4), Teaching hours/week (0), Practical Hours/week (0). The total course credits are 4.
For all other biotech 7th sem syllabus for b.tech 2017 regulation anna univ you can visit Biotech 7th Sem syllabus for B.Tech 2017 regulation Anna Univ Subjects. The detail syllabus for immunology laboratory is as follows.”
Course Objective:
- To give practical training in the functioning of immune system.
- To give laboratory training in different immunological and immunotechnological techniques.
List of Experiments:
- Identification of immune cells in a blood smear
- Identification of blood group
- Testing for typhoid antigens by Widal test
- Immunodiffusion – Ouchterlony Double Diffusion
- Immunoelectrophoresis – Rocket or Counter Current immunoelectrophoresis
- Enzyme Linked ImmunoSorbent Assay (ELISA)
- Isolation of peripheral blood mononuclear cells
- Isolation of monocytes from blood
- Immunofluorescence
- Identification of t cells by T-cell rossetting using sheep RBC.
Course Outcome:
- The students would be aware of immune system cells and tissues.
- The students would have knowledge on immunological /clinical tests.
- The students would be able to isolate lymphocytes and monocytes.
- The students would be able to identify various immune system cells.
List of Equipments for 30 Students
- Elisa reader 1
- Microscopes 8
- Microwave owen 1
- Hot plate 4
- Vortex mixer 4
- Table top refrigerated Centrifuge 1
- Fluorescent microsope 1
References:
- Roitt I, Male, Brostoff. Immunology, Mosby Publ., 2002.
- Kuby J, Immunology, WH Freeman and Co., 2000.
- Ashim K. Chakravarthy, Immunology, TataMcGraw-Hill, 1998.
For detail syllabus of all other subjects of B.Tech Biotech, 2017 regulation do visit Biotech 7th Sem syllabus for 2017 Regulation.
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