Downstream Processing Laboratory detail syllabus for Biotechnology (Biotech), 2017 regulation is taken from Anna University official website and presented for students of Anna University. The details of the course are: course code (BT8711), Category (PC), Contact Periods/week (4), Teaching hours/week (0), Practical Hours/week (0). The total course credits are 4.
For all other biotech 7th sem syllabus for b.tech 2017 regulation anna univ you can visit Biotech 7th Sem syllabus for B.Tech 2017 regulation Anna Univ Subjects. The detail syllabus for downstream processing laboratory is as follows.”
Course Objective:
- To provide hands on training in Down stream processing through simple experimentations in the laboratory. This will be a pre-requisite for project work.
- To understand the nature of the end product, its concentration, stability and degree of purification required
- To design processes for the recovery and subsequent purification of target biological products.
The objectives of this course is to practice the students
List of Experiments:
- Solid liquid separation – centrifugation
- Solid liquid separation – microfiltration
- Cell disruption techniques – ultrasonication or French pressure cell or Dynomill
- Precipitation – ammonium sulphite precipitation
- Ultra filtration separation
- Aqueous two phase extraction of biologicals
- High resolution purification – affinity chromatography
- High resolution purification – ion exchange chromatography
- Product polishing – spray drying or freeze drying
- Size exclusion chromatography
List of Equipment for 30 Students
- Centrifuge 1
- Cross flow filtration set up 2
- FPLC 1
- Sonicator or French press or Dynomill 1
Course Outcome:
Upon success completion of this course, the students would have
- Acquired knowledge for the separation of whole cells and other insoluble ingredients from the culture broth.
- Learned cell disruption techniques to release intracellular products
- Learned various techniques like evaporation, extraction, precipitation, membrane separation for concentrating biological products
- Learned the basic principles and techniques of chromatography to purify the biological products and formulate the products for different end uses.
References:
- P.A. Belter, E.L. Cussler And Wei-Houhu – Bioseparations – Downstream Processing For Biotechnology, Wiley Interscience Pun. (1988).
- R.O. Jenkins, (Ed.) – Product Recovery In Bioprocess Technology – Biotechnology ByOpen Learning Series, Butterworth-Heinemann (1992).
- J.C. Janson And L. Ryden, (Ed.) – Protein Purification – Principles, High ResolutionMethods And Applications, VCH Pub. 1989.
For detail syllabus of all other subjects of B.Tech Biotech, 2017 regulation do visit Biotech 7th Sem syllabus for 2017 Regulation.
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