Biochemistry Laboratory detailed syllabus for Pharmaceutical Technology (Pharma Tech) for 2019 regulation curriculum has been taken from the Anna Universities official website and presented for the Pharma Tech students. For course code, course name, number of credits for a course and other scheme related information, do visit full semester subjects post given below.
For Pharmaceutical Technology 2nd Sem scheme and its subjects, do visit Pharma Tech 2nd Sem 2019 regulation scheme. The detailed syllabus of biochemistry laboratory is as follows.
Course Objective:
The course aims to,
- learn and understand the principles behind the qualitative and quantitative estimation of biomolecules (proteins, carbohydrates, lipids, metabolites etc.,).
- make students to learn the basic units of measurements and standardisation of various buffer solutions
List of Experiments:
- General guidelines for working in biochemistry lab (theory)
- Units of volume, weight, density and concentration measurements and their range in biological measurements. Demonstration of proper use of volume and weight measurement devices.
- Accuracy, precision, sensitivity and specificity (theory)
- Preparation of buffer -titration of a weak acid and a weak base.
- Qualitative tests for carbohydrates – distinguishing reducing from non-reducing sugars and keto from aldo sugars.
- Quantitative method for amino acid estimation using ninhydrin – distinguishing amino from imino acid.
- Protein estimation by Biuret and Lowry”s methods.
- Protein estimation by Bradford and spectroscopic methods.
- Extraction of lipids and analysis by TLC.
- Estimation of nucleic acids by absorbance at 260 nm and hyperchromic effect (demo).
- Enzymatic assay: phosphatase from potato.
- Enzymatic assay: estimation of glucose by GOD-POD method after hydrolysis of starch with acid and specificity of the enzymatic method.
Equipment Needed for 20 Students
Autoclave 1
Hot Air Oven 1
Incubators 2
Light Microscopes 4
Incubator Shaker 1
Colorimeter 2
Laminar Flow Chamber 2
Glassware, Chemicals, Media as Required
Course Outcome:
At the end of the course the students will be able to,
- learn various qualitative and quantitative techniques
- describe various types of biochemical reaction
- evaluate the novelty of the experiment
Text Books:
- Practical Biochemistry by R.C. Gupta and S. Bhargavan. I Edition 2014.
- Introduction of Practical Biochemistry by David T. Plummer. III Edition, 2017
References:
- Harpers Biochemistry Ed. R.K. Murray, D.K. Granner, P.A. Mayes and V.W.Rodwell, Appleton and Lange ,Stanford ,Conneticut.24th edition 1996.
- Textbook of Biochemistry with clinical correlations. Ed. Thomas M. Devlin. Wiley Liss Publishers 6th edition 2015.
For detailed syllabus of all other subjects of Pharmaceutical Technology, 2019 regulation curriculum do visit Pharma Tech 2nd Sem subject syllabuses for 2019 regulation.
For all Pharmaceutical Technology results, visit Anna University Pharma Tech all semester results direct link.