Biochemistry Lab detailed syllabus for Industrial Technology (Industrial Biotech) for 2019 regulation curriculum has been taken from the Anna Universities official website and presented for the Industrial Biotech students. For course code, course name, number of credits for a course and other scheme related information, do visit full semester subjects post given below.
For Industrial Technology 3rd Sem scheme and its subjects, do visit Industrial Biotech 3rd Sem 2019 regulation scheme. The detailed syllabus of biochemistry lab is as follows.
Course Objective:
The course aims to
- learn and understand the principles behind the qualitative and quantitative estimation of biomolecules (proteins, carbohydrates, lipids, metabolites etc.,).
- make students learn the basic units of measurements and standardisation of various buffer solutions
List of Experiments:
- General guidelines for working in biochemistry lab (theory)
- Units of volume, weight, density and concentration measurements and their range in biological measurements. Demonstration of proper use of volume and weight measurement devices.
- Accuracy, precision, sensitivity and specificity (theory)
- Preparation of buffer -titration of a weak acid and a weak base.
- Qualitative tests for carbohydrates
- Quantitative method for amino acid estimation using Ninhydrin – distinguishing amino from imino acid.
- Protein estimation by Biuret and Lowry”s methods.
- Protein estimation by Bradford and spectroscopic methods.
- Extraction of lipids and analysis by TLC.
- Estimation of nucleic acids by absorbance at 260 nm and hyperchromic effect (demo).
- Enzymatic assay: phosphatase from potato.
- Enzymatic assay: estimation of glucose by GOD-POD
Equipment Needed for 20 Students
Autoclave 1
Hot Air Oven 1
Incubators 2
Light Microscopes 4
Incubator Shaker 1
Colorimeter 2
Laminar Flow Chamber 2
Glassware, Chemicals, Media as Required
Course Outcome:
At the end of the course the students will be able to
- know various qualitative and quantitative techniques.
- describe various types of biochemical reaction
- evaluate the novelty of the experiment
Text Books:
- Practical Biochemistry by R.C. Gupta and S. Bhargavan.5th edition, 2013.
- Introduction of Practical Biochemistry by David T. Phummer. (II Edition),1978.
References:
- Harpers Biochemistry Ed. R.K. Murray , D.K. Granner, P.A. Mayes and V.W.Rodwell, Appleton and Lange ,Stanford ,Conneticut,24th edition,1996
- Textbook of Biochemistry with clinical correlations. Ed. Thomas M. Devlin. Wiley Liss Publishers,6th edition,2006.
For detailed syllabus of all other subjects of Industrial Technology, 2019 regulation curriculum do visit Industrial Biotech 3rd Sem subject syllabuses for 2019 regulation.
For all Industrial Technology results, visit Anna University Industrial Biotech all semester results direct link.