IB5311: Cell Biology Lab Syllabus for Industrial Biotech 3rd Sem 2019 Regulation Anna University

Cell Biology Lab detailed syllabus for Industrial Technology (Industrial Biotech) for 2019 regulation curriculum has been taken from the Anna Universities official website and presented for the Industrial Biotech students. For course code, course name, number of credits for a course and other scheme related information, do visit full semester subjects post given below.

For Industrial Technology 3rd Sem scheme and its subjects, do visit Industrial Biotech 3rd Sem 2019 regulation scheme. The detailed syllabus of cell biology lab is as follows.

Course Objective:

The course aims to

• make the students learn about the principles of microscopy and sterilisation techniques
• train the students about different cell staining and viability methods

List of Experiments:

1. Introduction to principles of sterile techniques and cell propagation
2. Principles of microscopy , phase contrast and fluorescent microscopy
3. Identification of given plant, animal and bacterial cells and their components by microscopy
4. Staining methods
   1. Haematoxylin and Eosin
   2. Leishman staining
   3. Giemsa staining
5. Cell/tissue lysis and fractionation
   1. Homogenization
   2. Osmosis
   3. Differential centrifugation
6. Cell viability studies
   1. Tryphan blue dye exclusion
   2. Using tetrazolium salts

Course Outcome:

At the end of the course the students will be able to

1. develop the ability to propagate cells under in vitro conditions as well as their storage.
2. carryout differential staining in order to understand the internal components/complexities of a cell.
3. evaluate the integrity and lysis of cells in culture for downstream experiments

Equipment Required:

(For 60 Students)

• Micropipettes (0.5-2.5 pl) – 30 Nos.
• Micropipettes (2-20 pl) – 30 Nos.
• Micropipettes (20-200 pl) – 30 Nos.
• Micropipettes (100-1000 pl) – 30 Nos.
• Refrigerated centrifuge – 2 Nos.
• Temperature controlled Incubator shaker – 2 Nos.
• Temperature controlled water bath – 2 Nos.
• Ice flake machine – 2 Nos.
• Phase contrast microscope (Upright) – 8 Nos.
• Phase contrast microscope (Inverted) – 2 Nos.
• Tissue homogenizer – 2 Nos
• Microplate reader – 1 No.
• Glass wares/Plastic wares/Chemicals/Media as required

Text Books:

1. Rickwood, D. and J.R. Harris Cell Biology : Essential Techniques, John Wiley, 1996.
2. Davis, J.M. Basic Cell Culture : A Practical Approach, IRL, 1994.

For detailed syllabus of all other subjects of Industrial Technology, 2019 regulation curriculum do visit Industrial Biotech 3rd Sem subject syllabuses for 2019 regulation.

For all Industrial Technology results, visit Anna University Industrial Biotech all semester results direct link.