FT5212: Food Microbiology Laboratory Syllabus for Food Tech 2nd Sem 2019 Regulation Anna University

Food Microbiology Laboratory detailed syllabus for Food Technology (Food Tech) for 2019 regulation curriculum has been taken from the Anna Universities official website and presented for the Food Tech students. For course code, course name, number of credits for a course and other scheme related information, do visit full semester subjects post given below.

For Food Technology 2nd Sem scheme and its subjects, do visit Food Tech 2nd Sem 2019 regulation scheme. The detailed syllabus of food microbiology laboratory is as follows.

Course Objective:

The course aims to

• enable students to understand the methods of isolating and characterizing various microbes associated with foods and food groups.
• enable students to understand and use various microbiological techniques for the study of foods.
• Understand the methods used to detect pathogens in foods.

List of Experiments:

1. Introduction, Laboratory Safety, Use of Equipment; Sterilization Techniques; Culture Media-Types and Use; Preparation of Nutrient broth and agar
2. Culture Techniques, Isolation and Preservation of Cultures- Broth flask, test tubes; Solid Pour plates, streak plates, slants, stabs
3. Microscopy – Working and care of Microscope; Microscopic Methods in the Study of Microorganisms; Staining Techniques – Simple, Differential- Gram”s Staining
4. Quantification of Microbes Sampling and Serial Dilution; Bacterial count in food products TVC
5. Microbiological quality of water (MPN)
6. Microbiological quality of milk
7. Enumeration of Lactic acid bacteria from fermented foods
8. Yeast and Mould count from fruits
9. Enumeration of spores from pepper
10. Inhibitory effect of spices on microbial load in fish and flesh foods
11. Enumeration and Isolation of E. coli from processed meat/chicken
12. Thermal destruction of microbes TDT and TDP
13. Enumeration and Isolation of Staphylococci from ready to eat street foods
14. Effect of cleaning and disinfection on microbial load

Course Outcome:

At the end of the course the students will be able to

1. different techniques for the identification,isolation and culture of microbes.
2. analyse and identify microbial contamination in food
3. disinfection techniques

References:

1. Harrigan, W.F. “Laboratory Methods in Food Microbiology” Academic Press, 2011.

For detailed syllabus of all other subjects of Food Technology, 2019 regulation curriculum do visit Food Tech 2nd Sem subject syllabuses for 2019 regulation.

For all Food Technology results, visit Anna University Food Tech all semester results direct link.