Bioprocess Laboratory detailed syllabus for Biotechnology & Biochemical Engineering (BBE) for 2021 regulation curriculum has been taken from the Anna University official website and presented for the BBE students. For course code, course name, number of credits for a course and other scheme related information, do visit full semester subjects post given below.
For Biotechnology & Biochemical Engineering 6th Sem scheme and its subjects, do visit BBE 6th Sem 2021 regulation scheme. The detailed syllabus of bioprocess laboratory is as follows.
Course Objectives:
- To provide a practical exposure to the bioinformatics databases, tools and software.
- To select and use functions in Perl for processing sequence data.
Experiments
- Enzyme kinetics – Determination of Michaelis – Menten parameters
- Enzyme activity – Effect of Temperature and Deactivation Kinetics
- Enzyme activity – Effect of pH
- Enzyme inhibition kinetics
- Enzyme immobilization – Gel entrapment
- Enzyme immobilization -Cross-linking
- Enzymatic conversion in Packed bed Column
- Growth of Bacteria – Estimation of Biomass, Calculation of Specific Growth Rate, Yield Coefficient
- Optimization by Plackett Burman Design
- Optimization by Response Surface Methodology
- Estimation of KLa – Dynamic by different methods
- Estimation of Overall Heat Transfer Coefficient and mixing time in reactor
Course Outcomes:
At the end of this course, students will be able to:
- Explain about Enzyme kinetics and characterization and how to use them for practical applications.
- Evaluate the growth kinetics of microorganisms and become adept with medium optimization techniques.
- Determine an experimental objective, understand the theory behind the experiment, and operate the relevant equipment safely.
- Demonstrate good lab citizenry and the ability to work in team.
Reference Books:
- Peter F. Stanbury, Stephen J. Hall & A. Whitaker, Principles of Fermentation Technology, Butterworth-Heinemann; 3rd Edition ,2016.
- Pauline M. Doran, Bioprocess Engineering Principles, Elsevier, 2nd Edition,2012
- Michael L. Shuler, Fikret Kargi, Matthew De Lisa , Bioprocess Engineering , Prentice Hall, 3rd Edition, 2017
- Bailey, James E. and David F. Ollis, “ Biochemical Engineering Fundamentals”, 2ndEdition. McGraw Hill , 1986.
For detailed syllabus of all other subjects of Biotechnology & Biochemical Engineering, 2021 regulation curriculum do visit BBE 6th Sem subject syllabuses for 2021 regulation.
For all Biotechnology & Biochemical Engineering results, visit Anna University BBE all semester results direct link.