Plant Tissue Culture and Transformation Techniques detailed syllabus for Biotechnology (BioTech) for 2021 regulation curriculum has been taken from the Anna Universities official website and presented for the BioTech students. For course code, course name, number of credits for a course and other scheme related information, do visit full semester subjects post given below.
For Biotechnology 6th Sem scheme and its subjects, do visit BioTech 6th Sem 2021 regulation scheme. For Professional Elective-VIII scheme and its subjects refer to BioTech Professional Elective-VIII syllabus scheme. The detailed syllabus of plant tissue culture and transformation techniques is as follows.
Course Outcomes:
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Unit I
PLANT TISSUE CULTURE : 9
History of plant tissue culture research – basic principles of plant tissue callus culture, meristem culture, organ culture, Totipotency of cells, differentiation and dedifferentiation. Methodology -sterilization (physical and chemical methods), culture media, Murashige and Skoog’s (MS medium), phytohormones, medium for micro-propagation/clonal propagation of ornamental and horticulturally important plantsCallus subculture maintenance, growth measurements, morphogenesis
Unit II
PLANT TISSUE CULTURE 9
Endosperm culture – Embryo culture -culture requirements – applications, embryo rescue technique. Production of secondary metabolites. Cryopreservation; Germ plasm conservation
Unit III
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Unit IV
TISSUE CULTURE IN FOREST TREES 9
In vitro propagation via enhanced release of auxiliary buds. Somatic organogenesis and somatic embryo genesis, leaf diseases, embryoid and synthetic seed production. Haploid culture and production of homodiploids, Protoplast isolation, culture and regeneration.
Unit V
TRANSFORMATION TECHNIQUES : 9
Genetic transformation techniques in plants: Gene transfer methods in plants – Direct DNA transfer methods, Agro bacterium mediated nuclear transformation. Ti and Ri plasmids, binary & cointegrated vector systems; genetic markers; reporter genes; genetic transformation techniques for overcoming biotic and abiotic stress. Green house and green home technology. Arid and semiarid technology
Text Books:
- Botany-Plant tissue culture and its biotechnological applications, by B. R. C. Murthy & V. S.T. Sai, Venkateswara Publications, Gu
Reference Books:
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For detailed syllabus of all the other subjects of Biotechnology 6th Sem, visit BioTech 6th Sem subject syllabuses for 2021 regulation.
For all Biotechnology results, visit Anna University BioTech all semester results direct link.