BioTech

BT3027: Biotechniques in Animal Breeding syllabus for BioTech 2021 regulation (Professional Elective-V)

Biotechniques in Animal Breeding detailed syllabus for Biotechnology (BioTech) for 2021 regulation curriculum has been taken from the Anna Universities official website and presented for the BioTech students. For course code, course name, number of credits for a course and other scheme related information, do visit full semester subjects post given below.

For Biotechnology 6th Sem scheme and its subjects, do visit BioTech 6th Sem 2021 regulation scheme. For Professional Elective-V scheme and its subjects refer to BioTech Professional Elective-V syllabus scheme. The detailed syllabus of biotechniques in animal breeding is as follows.

Course Objectives:

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Unit I

BASIC TOOLS REQUIREMENTS FOR CELL CULTURE AND MICROMANIPULATION 9
Biosafety levels, safety equipments, personal protective equipments, safe laboratory practices. cell culture equipments: basic equipments – centrifuge, Inverted microscope, confocal microscope, flow cytometer, Hemocytometer, cell culture vessels, bioreactors. Cell culture laboratory: Aseptic work area, Cell culture hood, Incubator, cryostorage,cell counter, aseptic technique, Maintenance of nutrients, prevention of cross contamination. Micromanipulation tools: micromanipulator, pipette puller, pipette grinder, holding pipette,

Unit II

MICROMANIPULATION AND ITS APPLICATION 9
Enrichment of xand y bearing sperms from semen samples of animals; artificial insemination and germ cellmanipulations; in vitro fertilization and embryo transfer; micromanipulation technology and breeding of farm animals.

Unit III

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Unit IV

TRANSGENIC ANIMALS IN RESEARCH 9
Ethics of transgenic technology, Dolly (transgenic sheep), Transgenic mice, rat, sheep, goat, rabbit, pig, fish, cow- case studies.

Unit V

ETHICAL GUIDELINES ON ANIMAL BREEDING 9
Justification on research, care and housing of laboratory animals, acquisition of laboratory animals, experimental procedure, CPCSEA guidelines. Animal integrity and ethical limits to breeding. Animal welfare issues. Record Maintenance as per guidelines.

Course Outcomes:

Upon completion of the course the student will able to

  • Understand the concept of basic tools requirement for cell culture and micromanipulation
  • Gain knowledge on micromanipulation and its application
  • Know the concept of stem cells and ES cell of transgenic animals.
  • Understand the research importance in transgenic animals.
  • Gain knowledge on ethical CPCSEA guidelines

Reference Books:

Download the iStudy App for all syllabus and other updates.
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For detailed syllabus of all the other subjects of Biotechnology 6th Sem, visit BioTech 6th Sem subject syllabuses for 2021 regulation.

For all Biotechnology results, visit Anna University BioTech all semester results direct link.

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