Animal Cell Culture Technology detailed syllabus for Biotechnology (BioTech) for 2021 regulation curriculum has been taken from the Anna Universities official website and presented for the BioTech students. For course code, course name, number of credits for a course and other scheme related information, do visit full semester subjects post given below.
For Biotechnology 6th Sem scheme and its subjects, do visit BioTech 6th Sem 2021 regulation scheme. For Professional Elective-V scheme and its subjects refer to BioTech Professional Elective-V syllabus scheme. The detailed syllabus of animal cell culture technology is as follows.
Course Objectives:
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Unit I
BASIC REQUIREMENTS OF LAB FACILITY 9
Safety: biosafety levels, SDS, safety equipments, personal protective equipments, safe laboratory practices.cell culture equipments: basic equipments – centrifuge, Inverted microscope, confocal microscope, flow cytometer, Hemocytometer, cell culture vessels, bioreactors. Cell culture laboratory: Aseptic work area, Cell culture hood, Incubator, cryostorage,cell counter, aseptic technique, Maintenance of nutrients, prevention of cross contamination.
Unit II
MEDIA PREPARATION AND TYPES 9
Media components-Serum, tissue extracts, growth factors, hormones, carrier proteins, lipids, vitamins, additive, detergents. Types: natural media, synthetic media, chemically defined and serum free media – advantages, disadvantages, BSS, CMRL, Eagle’s, RPMI, animalcell cultures, their maintenance and preservation;
Unit III
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Unit IV
GENETIC ENGINEERING OF ANIMAL CELL 9
Gene therapy-prospects and problems, Recent advancements in Gene therapy; Knock out mice and mice model for human genetic disorder; Baculo virus in biocontrol; Enzymes technology, Somatic manipulation of DNA, Nucleic acid hybridization and probes in diagnosis- preparation of probes, evaluation and applications.Recent advancements in diagnostic tool development and its diagnostic procedure
Unit V
PRODUCTS FROM ANIMAL CELL 9
Enzymes – asperagenase, collagenase, urokinase, pepsin, hyaluronidase. Hormones- leutinizing hormones, FSH, chronic.Vaccines – FMD, measles and mumps, rubella, rabies monoclonal antibodies, interferons, plasminogen activator.
Course Outcomes:
Upon completion of this subject the student will be able to
- Understand the basic requirements of lab facility
- Know the various types of media
- Understand the bioreactor and growth of cells
- Learn the role of genetic engineering in animal cell culture
- Understand the valuable products from animal cell
Reference Books:
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For detailed syllabus of all the other subjects of Biotechnology 6th Sem, visit BioTech 6th Sem subject syllabuses for 2021 regulation.
For all Biotechnology results, visit Anna University BioTech all semester results direct link.