Biosciences Laboratory detailed syllabus for Biomedical Engineering (BME) for 2021 regulation curriculum has been taken from the Anna University official website and presented for the BME students. For course code, course name, number of credits for a course and other scheme related information, do visit full semester subjects post given below.
For Biomedical Engineering 2nd Sem scheme and its subjects, do visit BME 2nd Sem 2021 regulation scheme. The detailed syllabus of biosciences laboratory is as follows.
Course Objectives:
To provide practice on:
- Estimation and quantification of biomolecules.
- Separation of macromolecules.
- Use Compound microscope
- Practice on chemical examinations, Histopathological examinations etc
List of Experiments:
- Preparation of solutions: a) percentage solutions, a) molar solutions, a) normal solutions
- Standardization of pH meter, preparation of buffers, emulsions.
- Spectroscopy: Determination of absorption maxima (Amax) of a given solution
- General tests for carbohydrates, proteins and lipids.
- Identification of Blood Collection Tubes and Phlebotomy equipment
- Preparation of serum and plasma from blood
- Estimation of Haemoglobin and blood glucose
- Estimation of creatinine, urea and Uric acid
- Separation of proteins by SDS electrophoresis(Demo) and amino acids by thin layer chromatography (Demo).
- Urine physical and chemical examination (protein, reducing substances, ketones, bilirubin and blood)
- Basic staining – Hematoxylin and eosin staining.
- Special stains – cresyl fast Blue (CFV)- Trichrome – oil red O – PAS
- Types of Staining : Simple stain, Gram stain
- Study of parts of compound microscope
- Study of Histopathological slides of benign and malignant tumours.
- Study of Haematology slides of anemia and leukemia.
Course Outcomes:
Upon completion of the course, students will be able to:
- Understand the Biochemistry laboratory functional components
- Have a sound knowledge of qualitative test of different biomolecules.
- Understand the basics knowledge of Biochemical parameter and their interpretation in Blood sample.
- Have a sound knowledge of separation technology of proteins and amino acids.
- Student can perform practical experiments on staining Processes.
Text Books:
- Ramnik Sood, Textbook of Medical Laboratory Technology, 6thEdition, Jaypee Brothers Medical Publishers, 2009
Lab Requirement for a Batch of 30 Students:
- Requirement for a batch of 30 students
- Colorimeter 2 Nos
- Spectrophotometer 1 No.
- pH meter 1 No
- Weighing balance 1 No
- Refrigerator 1 No
- SDS gel electrophoresis 1 No TLC, ready TLC plates 1 No Wintrobes tube 2 Nos.
- Centrifuge Normal 1 No
- Microslides 2 packets
- Lancet 5 boxes
- Microscope 1 No
- Neubaurs Chamber 2 Nos.
- Heparinized Syringe 1box
- Haemoglobinometer 1 No Capillary tubes 1 box
For detailed syllabus of all other subjects of Biomedical Engineering, 2021 regulation curriculum do visit BME 2nd Sem subject syllabuses for 2021 regulation.
For all Biomedical Engineering results, visit Anna University BME all semester results direct link.