Biochemistry Laboratory detail syllabus for Pharmacy (Pharma), 2017 regulation is taken from Anna University official website and presented for students of Anna University. The details of the course are: course code (PY8211), Category (PC), Contact Periods/week (4), Teaching hours/week (0), Practical Hours/week (0). The total course credits are 4.
For all other pharma 2nd sem syllabus for b.tech 2017 regulation anna univ you can visit Pharma 2nd Sem syllabus for B.Tech 2017 regulation Anna Univ Subjects. The detail syllabus for biochemistry laboratory is as follows.”
Aims:
- To learn and understand the principles behind the qualitative and quantitative estimation of biomolecules (proteins, carbohydrates, lipids, metabolites etc.,) and laboratory analysis of the same in the body fluids.
List of Experiments:
- General guidelines for working in biochemistry lab (theory)
- Units of volume, weight, density and concentration measurements and their range in biological measurements. Demonstration of proper use of volume and weight measurement devices.
- Accuracy, precision, sensitivity and specificity (theory)
- Preparation of buffer -titration of a weak acid and a weak base.
- Qualitative tests for carbohydrates – distinguishing reducing from non-reducing sugars and keto from aldo sugars.
- Quantitative method for amino acid estimation using ninhydrin – distinguishing amino from imino acid.
- Protein estimation by Biuret and Lowrys methods.
- Protein estimation by Bradford and spectroscopic methods.
- Extraction of lipids and analysis by TLC.
- Estimation of nucleic acids by absorbance at 260 nm and hyperchromic effect (demo).
- Enzymatic assay: phosphatase from potato.
- Enzymatic assay: estimation of glucose by GOD-POD method after hydrolysis of starch with acid and specificity of the enzymatic method.
Equipment Needed for 20 Students
- Autocalve 1
- Hot Air Oven 1
- Incubators 2
- Light Microscopes 4
- Incubator Shaker 1
- Colorimeter 2
- Laminar Flow Chamber 2
- Glassware, Chemicals, Media as required
Text Books:
- Practical Biochemistry by R.C. Gupta and S. Bhargavan.
- Introduction of Practical Biochemistry by David T. Phummer. (II Edition)
References:
- Harpers Biochemistry Ed. R.K. Murray , D.K. Granner, P.A. Mayes and V.W.Rodwell, Appleton and Lange ,Stanford ,Conneticut.
- Textbook of Biochemistry with clinical correlations. Ed. Thomas M. Devlin. Wiley Liss Publishers
For detail syllabus of all other subjects of B.Tech Pharma, 2017 regulation do visit Pharma 2nd Sem syllabus for 2017 Regulation.
Dont forget to download iStudy for latest syllabus and results, class timetable and more.