Biochemistry and Physiology Laboratory detailed syllabus for Pharmacy (Pharma) for 2021 regulation curriculum has been taken from the Anna University official website and presented for the Pharma students. For course code, course name, number of credits for a course and other scheme related information, do visit full semester subjects post given below.
For Pharmacy 3rd Sem scheme and its subjects, do visit Pharma 3rd Sem 2021 regulation scheme. The detailed syllabus of biochemistry and physiology laboratory is as follows.
Course Objectives:
The course aims the students to,
- Learn and understand the principles behind the qualitative and quantitative estimation of biomolecules (proteins, carbohydrates, lipids, metabolites etc.,).
- To learn the gross histology, structure and functions of various organs of the human body and perform the physiological tests and appreciate the interlinked mechanisms in the maintenance of normal functioning of human body
List of Experiments:
Biochemistry
- Qualitative tests for carbohydrates-distinguishing reducing from non-reducing sugars and keto from aldo sugars.
- Quantitative method for aminoacid estimation usingbninhydrin-distinguishing amino from imino acid.
- Quantitative analysis of carbohydrates (Benedicts method etc.,)
- Protein estimation by Biuret, Lowrys method, Bradford and spectroscopic methods
- Extraction of lipids and analysis byTLC.
- Enzymatic assay:phosphatase from potato.
- Enzymatic assay: estimation of glucose by GOD-POD method.
- Estimation of chloride, glucose, ammonia and creatinine in urine.
Physiology
- Study of different systems with the help of models (axial skeleton, appendicular skeleton, cardiovascular system, respiratory system, digestive system, urinary system, nervous system, special senses, reproductive system)
- Determination of bleeding and clotting time
- Determination of R.B.C. and W.B.C count of blood
- Estimation of Haemoglobin
- Determination of differential count of blood WBCs.
- Enumeration of RBC
- Determination of Erythrocyte Sedimentation Rate
- Blood group determination
- Observation of osmotic fragility of RBCs
- Determination of packed cell volume and calculation of blood indices
- Heart rate and blood pressure recording
- ECG recording
- Examination of respiratory system and recording of respiratory movements
- Preparation of physiological solution: Ringers solution, Tyrode solution, Krebs solution, Normal saline.
Course Outcomes:
On completion of the course, the students would be able to
- Apply basic principles of chemistry to biological systems and molecular biology.
- Correlate molecular structure and interactions present in proteins, nucleic acids, carbohydrates and lipids
- Perform procedure to characterize the biomolecules using microscopy.
- Understand the gross histology, structure and functions of various organs of the human body
- Implement the physiological tests and appreciate the interlinked mechanisms in the maintenance of normal functioning of human body
- Perform basic physiological and pharmacological experiments and to record and interpret the results for its clinical significance.
Reference Books:
- Human anatomy & physiology laboratory manual. Cat version Marieb, Elaine Nicpon, Mitchell, Susan J., Smith, Lori A., Zao, Peter Z. Year: 2016 Edition: Twelfth edition, cat version. Publisher: Pearson
- Laboratory Manual for Anatomy & Physiology Featuring Martini Art, Cat Version Year: 2016 Edition: 6th Edition Publisher: Pearson
- Practical Biochemistry with Clinical Correlation for MBBS Students, Poonam Agrawal. Year: 2020, Edition: 1, Publisher: CBS Publishers and Distributors Pvt Ltd
- Practical Biochemistry for Colleges, E. J. Wood (Eds.) Year: 1989 Edition: 1st ed Publisher: Pergamon Press
- Experimental Biochemistry, Robert L. Switzer, Liam F. Garrity Year: 1999 Edition: 3rd ed Publisher: W. H. Freeman and Co
- Principles and techniques of practical biochemistry and molecular biology. Keith Wilson, John Walker Year: 2005 Edition
For detailed syllabus of all other subjects of Pharmacy, 2021 regulation curriculum do visit Pharma 3rd Sem subject syllabuses for 2021 regulation.
For all Pharmacy results, visit Anna University Pharma all semester results direct link.