Microbiology Laboratory detailed syllabus for Pharmacy (Pharma) for 2021 regulation curriculum has been taken from the Anna University official website and presented for the Pharma students. For course code, course name, number of credits for a course and other scheme related information, do visit full semester subjects post given below.
For Pharmacy 3rd Sem scheme and its subjects, do visit Pharma 3rd Sem 2021 regulation scheme. The detailed syllabus of microbiology laboratory is as follows.
Course Objectives:
- To practically demonstrate various experimental techniques to identify the morphology, culture characteristics, propagation and control of microbes and industrial applications of microbes.
List of Experiments:
- Demonstration of efficiency of sterilization techniques.
- Preparation of various types of culture media: nutrient broth and agar
- Culture techniques: isolation and preservation of cultures: broth: flask, test tubes; agar: pour plates, streak plates, slants, stabs
- Microscopic identification of bacteria
- Staining techniques: simple, differential- grams staining, spore /capsule staining
- Quantification of microbes: sampling and serial dilution; bacterial count, total count and viable count.
- Determination of phenol coefficient to demonstrate efficiency of a disinfectant
- Perform and report sensitivity of antibiotics (Disc diffusion and well diffusion)
- Inoculate, Incubate and plot the growth curve of bacteria (E.coli)
- Effect of pH, Temperature, UV radiation on Growth of bacteria (E.coli)
- Determination of microbial inhibitory concentration (MIC).
- Biochemical identification of unknown microbes
- Demonstration of alcohol fermentation.
Course Outcomes:
Students will be able to
- Understand the advanced technical information pertaining to laboratory bio-safety and preventive measures from pathogenic microorganism.
- Develop the minimum skills to work on several important techniques using equipment for the study of microorganisms. Microscopically examine living microorganisms.
- Familiar with the types of laboratory equipment and culture media needed to develop and maintain pure cultures.
- Carry out the technique for aseptic removal and transfer of microorganisms for subculturing.
- Determine the cultural characteristics of microorganisms as an aid in identifying and classifying organisms into taxonomic groups.
- Practical use of the compound microscope for visualization of cellular morphology from stained slide preparations.
Equipment Needed for 1 Batch
- Autoclave 2
- Hot Air Oven 2
- Incubators 2
- Light Optical Microscopes 6
- Incubator Shaker 2
- Colorimeter 2
- Lamina Flow Chamber 2
- Colony counter 2
- Laminar air flow 2
Reference Books:
- Practical Handbook of Microbiology, Second edition, Edited by Emanuel Goldman, Lorrence H Green, CRC Press.
- Cappuccino, J.G. and N. Sherman Microbiology: A Laboratory Manual, Global Edition, Pearson, 2018.
- Collee, J.G. etal., Mackie & Mc Cartney Practical Medical Microbiology 4th Edition, Churchill Livingstone, 1996.
For detailed syllabus of all other subjects of Pharmacy, 2021 regulation curriculum do visit Pharma 3rd Sem subject syllabuses for 2021 regulation.
For all Pharmacy results, visit Anna University Pharma all semester results direct link.