Cell and Molecular Biology Laboratory detailed syllabus for Pharmaceutical Technology (Pharma Tech) for 2019 regulation curriculum has been taken from the Anna Universities official website and presented for the Pharma Tech students. For course code, course name, number of credits for a course and other scheme related information, do visit full semester subjects post given below.
For Pharmaceutical Technology 4th Sem scheme and its subjects, do visit Pharma Tech 4th Sem 2019 regulation scheme. The detailed syllabus of cell and molecular biology laboratory is as follows.
Course Objective:
The course aims to,
- understand the principle of nucleic acid isolation.
- understd the principles of PCR and their uses in genetic engineering.
- gain a thorough knowledge about nucleic acid hybridization.
- learn the history of DNA sequencing and current methods and gene synthesis
List of Experiments:
- Preparation of Genomic DNA
- PCR amplification of gene from the genomic DNA
- Preparation of plasmid DNA
- Detection of Plasmid DNA by Agarose gel electrophoresis
- Restriction Digestion of the vector and Insert
- Ligation and Transformation ofE.coli
- Lysate PCR confirmation.
- Restriction and gel elution of DNA fragments
- Electroporation of Yeast
- SDS-PAGE analysis of purified protein
- Western blot confirmation of expressed protein (anti his)
- Quantification of proteins.
- RNA Isolation
- cDNA preparation from RNA
- Site directed mutagenesis
- Southern hybridization experiment
List of Equipments
REQUIRED FOR 30 STUDENTS
- PCR machine
- Electrophoretic assemblies for DNA and protein separations
- ELISA reader
- Ultracentrifuge
- Laminar air flow cabinets
- Cooling centrifuge
Course Outcome:
At the end of the course the students will be able to
- experience basic techniques of DNA isolation and manipulation
- experience in selecting genetically transformed organisms for downstream analysis
- experience basic techniques involved in analysis of gene expression at nucleic acids and proteins level
References:
- Old RW, Primrose SB, “Principles of Gene Manipulation, “An Introduction to Genetic Engineering”, Blackwell Science Publications, 1993.
- Ausubel FM, Brent R, Kingston RE, Moore DD, “Current Protocols in Molecular Biology, “Greene Publishing Associates, NY, 2003.
- S. John Venison, “Laboratory manual for Genetic Engineering”, Eastern economy edition, 2009.
For detailed syllabus of all other subjects of Pharmaceutical Technology, 2019 regulation curriculum do visit Pharma Tech 4th Sem subject syllabuses for 2019 regulation.
For all Pharmaceutical Technology results, visit Anna University Pharma Tech all semester results direct link.