Molecular Biology Laboratory detail syllabus for Biotechnology (Biotech), 2017 regulation is taken from Anna University official website and presented for students of Anna University. The details of the course are: course code (BT8412), Category (PC), Contact Periods/week (4), Teaching hours/week (0), Practical Hours/week (0). The total course credits are 4.
For all other biotech 4th sem syllabus for b.tech 2017 regulation anna univ you can visit Biotech 4th Sem syllabus for B.Tech 2017 regulation Anna Univ Subjects. The detail syllabus for molecular biology laboratory is as follows.”
Course Objective:
- Provide hands-on experience in performing basic molecular biology techniques.
- Introduce students to the theory behind in each technique and to describe common applications of each methodology in biological research. This will facilitate the students to take up specialized project in Molecular biology and will be a pre-requisite for research work.
List of Experiments:
- Electrophoresis _ Agarose and Polyacrylamide Gel
- Isolation of microbial DNA
- Isolation of genomic DNA
- Quantification of DNA (UV/ Vis) and analysis of purity
- Restriction enzyme digestion& Ligation
- Competent cells preparation
- Transformation
- Selection of recombinants – Antibiotic sensitivity assay
- Plating of A phage
- Lamda phage lysis of liquid cultures
Equipment Needed for 30 Students
- Electrophoresis Kit 1
- PCR 1
- Incubators 2
- Light Microscopes 4
- Incubator Shaker 1
- Spectrophotometer 2
- Laminar Flow Chamber 2
- Glassware, Chemicals, Media as required
Course Outcome:
By the end of this course, students should be able to:
- Demonstrate knowledge and understanding of the principles underpinning important techniques in molecular biology.
- Demonstrate knowledge and understanding of applications of these techniques.
- Demonstrate the ability to carry out laboratory experiments and interpret the results.
- Students will be aware of the hazardous chemicals and safety precautions in case of emergency
References:
- Sambrook, Joseph and David W. Russell The Condensed Protocols: From Molecular Cloning: A Laboratory Manual Cold Spring Harbor , 2006.
For detail syllabus of all other subjects of B.Tech Biotech, 2017 regulation do visit Biotech 4th Sem syllabus for 2017 Regulation.
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