Molecular Biology and Genetic Engineering Laboratory Pharma 4th Sem Syllabus for B.Tech 2017 Regulation Anna Univ

Molecular Biology and Genetic Engineering Laboratory detail syllabus for Pharmacy (Pharma), 2017 regulation is taken from Anna University official website and presented for students of Anna University. The details of the course are: course code (PY8412), Category (PC), Contact Periods/week (4), Teaching hours/week (0), Practical Hours/week (0). The total course credits are 4.

For all other pharma 4th sem syllabus for b.tech 2017 regulation anna univ you can visit Pharma 4th Sem syllabus for B.Tech 2017 regulation Anna Univ Subjects. The detail syllabus for molecular biology and genetic engineering laboratory is as follows.”

Course Objective:

• To understand the principle of nucleic acid isolation.
• To understand the principles of PCR and their uses in genetic engineering.
• To gain a thorough knowledge about nucleic acid hybridization.
• To learn history of DNA sequencing and current methods and gene synthesis

List of Experiments:

1. Preparation of Genomic DNA
2. PCR amplification of gene from the genomic DNA
3. Preparation of plasmid DNA
4. Detection of Plasmid DNA by Agarose gel electrophoresis
5. Restriction Digestion of the vector and Insert
6. Ligation and Transformation ofE.coli
7. Lysate PCR confirmation.
8. Restriction and gel elution of DNA fragments
9. Electroporation of Yeast
10. SDS-PAGE analysis of purified protein
11. Western blot confirmation of expressed protein (anti his)
12. ELISA- Quantification of proteins.
13. RNA Isolation
14. cDNA preparation from RNA
15. Site directed mutagenesis
16. Southern hybridization experiment
• PCR machine
• Electrophoretic assemblies for DNA and protein separations
• ELISA reader
• Ultracentrifuge
• Laminar air flow cabinets
• Cooling centrifuge

Course Outcome:

By the end of this course, students will be able to:

• Describe the main principles, methods for preparation and cloning of DNA in various organisms.
• Express clearly about the gene amplification and methods for analysis of DNA, such as hybridization, restriction analysis and gene expressions.
• Express clearly about the analysis of protein expressions.

References:

1. Old RW, Primrose SB, Principles of Gene Manipulation, An Introduction to GeneticEngineering , Blackwell Science Publications, 1993.
2. Ansubel FM, Brent R, Kingston RE, Moore DD, Current Protocols in Molecular Biology, Greene Publishing Associates, NY, 2003
3. S. John Vennison, Laboratory manual for Genetic Engineering, Eastern economy edition, 2009.

For detail syllabus of all other subjects of B.Tech Pharma, 2017 regulation do visit Pharma 4th Sem syllabus for 2017 Regulation.

Dont forget to download iStudy for latest syllabus and results, class timetable and more.