Downstream Processing Lab detailed syllabus for Industrial Technology (Industrial Biotech) for 2019 regulation curriculum has been taken from the Anna Universities official website and presented for the Industrial Biotech students. For course code, course name, number of credits for a course and other scheme related information, do visit full semester subjects post given below.
For Industrial Technology 7th Sem scheme and its subjects, do visit Industrial Biotech 7th Sem 2019 regulation scheme. The detailed syllabus of downstream processing lab is as follows.
Course Objective:
The course aims to
- understand the principles behind various separation, concentration and purification processes used in Bioprocess industry
- impart knowledge on various cell disruption techniques
- have a hands-on experience on various downstream processing steps involved in a bioprocess industry.
List of Experiments:
- Solid liquid separation – centrifugation, microfiltration
- Cell disruption techniques – ultrasonication, French pressure cell
- Cell disruption techniques – dynomill – batch and continuous
- Precipitation – ammonium sulphite precipitation
- Ultra filtration separation
- Aqueous two phase extraction of biologicals
- High resolution purification – affinity chromatography
- High resolution purification – ion exchange chromatography
- Product polishing – spray drying, freeze drying
List of Equipment for 30 Students
Centrifuge 1
Cross Flow Filtration Set Up 2
FPLC 1
Sonicator 1
French Press 1
Course Outcome:
At the end of the course the students will be able to
- extract intra and extra cellular proteins from biological samples by performing cell disruption using mechanical and enzymatic methods
- fractionate proteins using precipitation methods
- separate proteins using chromatographic techniques
- analyze the purity of proteins
- design processes for the recovery and subsequent purification of target biological products
References:
- Belter, P.A., E.L. Cussler and Wei-Houhu Bioseparations – Downstream Processing for Biotechnology, John Wiley, 1988
- R.O. Jenkins, (Ed.) – Product Recovery In Bioprocess Technology – Biotechnology By Open Learning Series, Butterworth-Heinemann, 2004.
- J.C. Janson And L. Ryden, (Ed.) – Protein Purification – Principles, High Resolution Methods And Applications, VCH Pub. 1989.
For detailed syllabus of all other subjects of Industrial Technology, 2019 regulation curriculum do visit Industrial Biotech 7th Sem subject syllabuses for 2019 regulation.
For all Industrial Technology results, visit Anna University Industrial Biotech all semester results direct link.