Molecular Biology and Genetic Engineering Lab detailed syllabus for Industrial Technology (Industrial Biotech) for 2019 regulation curriculum has been taken from the Anna Universities official website and presented for the Industrial Biotech students. For course code, course name, number of credits for a course and other scheme related information, do visit full semester subjects post given below.
For Industrial Technology 6th Sem scheme and its subjects, do visit Industrial Biotech 6th Sem 2019 regulation scheme. The detailed syllabus of molecular biology and genetic engineering lab is as follows.
Course Objective:
The course aims to
- make the students to learn the basic DNA isolation techniques.
- learn about the identification and characterisation of gene and protein
List of Experiments:
- Isolation of total DNA
- Isolation of Plasmid DNA
- Agarose gel electrophoresis
- Restriction enzyme digestion of DNA
- DNA ligation
- Competent cell preparation and Transformation
- Blue-White screening
- Induction and Analysis of Gene expression
- PCR
- SDS-PAGE
- Western blot
Equipment Required:
(for 60 students)
- Micropipettes (0.5-2.5 pl) – 30 Nos.
- Micropipettes (2-20 pl) – 30 Nos.
- Micropipettes (20-200 pl) – 30 Nos.
- Micropipettes (100-1000 pl) – 30 Nos.
- Refrigerated centrifuge – 2 Nos.
- Chemical fume hoods (for handling toxic solvents) – 2 Nos.
- Temperature controlled Incubator shaker – 2 Nos.
- Temperature controlled water bath – 2 Nos.
- Ice flake machine – 2 Nos.
- Agarose gel apparatus with power packs – 30 Nos.
- Laminar Air flow (3 or 4 ft length) – 2 Nos.
- PCR machine (96/48 Wells) – 2 Nos.
- SDS-PAGE apparatus – 30 Nos.
- Western transfer apparatus (Semi dry) – 2 Nos.
- Western transfer apparatus (wet) – 30 Nos.
- Glasswares / Plasticwares/Chemicals/Media as required
Course Outcome:
At the end of the course the students will be able to
- experience basic techniques of DNA isolation and manipulation
- experience in selecting genetically transformed organisms for downstream analysis.
- experience basic techniques involved in analysis of gene expression at nucleic acids and proteins level
References:
- Green MR and Sambrook J. Molecular Cloning: A Laboratory Manual. 4th Edition, CSHL press, 2012.
For detailed syllabus of all other subjects of Industrial Technology, 2019 regulation curriculum do visit Industrial Biotech 6th Sem subject syllabuses for 2019 regulation.
For all Industrial Technology results, visit Anna University Industrial Biotech all semester results direct link.