Genetic Engineering detailed syllabus for Industrial Technology (Industrial Biotech) for 2019 regulation curriculum has been taken from the Anna Universities official website and presented for the Industrial Biotech students. For course code, course name, number of credits for a course and other scheme related information, do visit full semester subjects post given below.
For Industrial Technology 6th Sem scheme and its subjects, do visit Industrial Biotech 6th Sem 2019 regulation scheme. The detailed syllabus of genetic engineering is as follows.
Course Objective:
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Unit I
Introduction To Recombinant Dna Technology
Overview of recombinant DNA technology and its applications. Recombinant DNA technology tools – Restriction and Modifying systems: Biological importance, Classification, Nomenclature, Applications in recombinant DNA technology – Cohesive ends, blunt ends, Isoschizomers, Neoschizomers, Star activity, Compatible cohesive ends, DNA polymerase, DNA ligase, Alkaline phosphatase – Inter and intra molecular ligation, Polynucleotide kinase, Terminal transferase and Exonuclease, Linkers and adpators.
Unit II
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Unit III
Dna Libraries
Construction of genomic and cDNA library: Methods, Chromosomal walking, Limitations in cDNA library construction and full-length cDNA library construction. Screening of DNA libraries: Nucleic acid hybridization and PCR – degenerate probes and primers, Southwestern and Northwestern strategies, Immunochemical, protein-protein/ligand interaction, functional complementation/gain of functionapproaches. Differential cDNA library: Differential expression analysis and screening, Subtracted cDNA library, PCR based differential display analysis and difference cloning.
Unit IV
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Unit V
Applications of Recombinant Dna Technology
Site directed mutagenesis: Primer extension method, Kunkel”s method and PCR based site-directed mutagenesis. Creation of transgenic animals and plants: Zinc finger, TALENS, meganucleases, CRISPR-Cas. DNA microinjection, Viral vectors, Transformation of embryonic stem cells and Ti plasmids. Applications of site specific mutants, transgenic animals and plants.
Course Outcome:
For the complete syllabus, results, class timetable, and many other features kindly download the iStudy App
It is a lightweight, easy to use, no images, and no pdfs platform to make students’s lives easier..
Text Books:
- Old RW, Primrose SB, Principles Of Gene Manipulation, An Introduction To Genetic Engineering , Blackwell Science Publications, 1993.
- Principles of Genome Analysis and Genomics by S.B.Primrose and R.M.Twyman, Third Edition (Blackwell Publishing),7th edition,2006
References:
- Primrose SB and Twyman RB. Principles of Gene manipulation and Genomics. 7th Edition, Wiley-Blackwell,2006.
- Green MR and Sambrook J. Molecular Cloning: A Laboratory Manual. 4th Edition, CSHL press,2012.
- Brown TA. Gene Cloning and DNA analysis: An introduction. 6th Edition, Wiley-Blackwell,2010.
For detailed syllabus of all other subjects of Industrial Technology, 2019 regulation curriculum do visit Industrial Biotech 6th Sem subject syllabuses for 2019 regulation.
For all Industrial Technology results, visit Anna University Industrial Biotech all semester results direct link.