Bioprocess Laboratory I detailed syllabus for Industrial Technology (Industrial Biotech) for 2019 regulation curriculum has been taken from the Anna Universities official website and presented for the Industrial Biotech students. For course code, course name, number of credits for a course and other scheme related information, do visit full semester subjects post given below.
For Industrial Technology 5th Sem scheme and its subjects, do visit Industrial Biotech 5th Sem 2019 regulation scheme. The detailed syllabus of bioprocess laboratory i is as follows.
Course Objective:
The course aims to
- design and set up experiments for optimizing process conditions at lab-scale for fermentation and enzymatic biotransformation processes
- understand the kinetics of enzymatic reactions
List of Experiments:
- Enzyme kinetics – Determination of Michaelis Menten parameters
- Enzyme activity – Effect of Temperature and Deactivation Kinetics
- Enzyme activity – Effect of pH
- Enzyme inhibition kinetics
- Enzyme immobilization – Gel entrapment/ Cross linking
- Enzymatic conversion in Packed bed Column/Fluidized bed Column
- Growth of Bacteria – Estimation of Biomass, Calculation of Specific Growth Rate, Yield Coefficient
- Growth of Algae – Estimation of Biomass, Calculation of Specific Growth Rate, Yield Coefficient
- Medium optimization – Plackett Burman Design
- Medium optimization – Response Surface Methodology
Course Outcome:
At the end of the course the students will be able to
- gain experience in enzyme kinetics and immobilized enzyme studies
- observe the growth kinetics of bacteria and algae
- understand the basic media optimizing strategies
Equipment Needed for 20 Students
Autoclave 1
Hot Air Oven 1
Incubators 2
Light Microscopes 4
Incubator Shaker 1
Colorimeter 2
Laminar Flow Chamber 2
Glassware, Chemicals,
Media as Required
References:
- Bailey and Ollis, Biochemical Engineering Fundamentals, McGraw Hill (2nd Ed.), 1986.
- Shuler and Kargi, Bioprocess Engineering , Prentice Hall, 1992.
- Pauline Doran, Bioprocess Engineering Calculation, Blackwell Scientific Publications,2012
- Peter F. Stanbury, Stephen J. Hall and A. Whitaker, Principles of Fermentation Technology, Science and Technology Books. 2nd edition 2008
For detailed syllabus of all other subjects of Industrial Technology, 2019 regulation curriculum do visit Industrial Biotech 5th Sem subject syllabuses for 2019 regulation.
For all Industrial Technology results, visit Anna University Industrial Biotech all semester results direct link.