Immunology Lab detailed syllabus for Industrial Technology (Industrial Biotech) for 2019 regulation curriculum has been taken from the Anna Universities official website and presented for the Industrial Biotech students. For course code, course name, number of credits for a course and other scheme related information, do visit full semester subjects post given below.
For Industrial Technology 5th Sem scheme and its subjects, do visit Industrial Biotech 5th Sem 2019 regulation scheme. The detailed syllabus of immunology lab is as follows.
Course Objective:
The course aims to
- learn the isolation and staining of different blood cells
- learn the different immunological assays
List of Experiments:
- Selection of animals and introduction to animal handling (mice/rat/rabbit/ chicken/fish etc)
- Determination of various routes of immunization
- Methods of bleeding, serum separation and storage
- Preparation of antigens/immunization schedule for raising antisera.
- Identification of leukocytes from blood smear by differential staining (Geimsa stain)
- Enumeration of RBCs/leukocytes by Neubar chamber for cell culture studies.
- Separation of Peripheral Blood Mononuclear Cells (PBMC) by Ficoll -Hypaque
- Determination of agglutination reaction: Blood grouping antigens
- Determination of immunoprecipitaion reaction: immunodiffusion/immunoelectrophoresis
- Evaluation of antibody titre by ELISA method
- Diagnostic determination of agglutination: Widal test
- Rapid diagnostic tests: Immunochromatographic test strips*Animal experiments will be conducted by trained faculties in accordance with UGC requirements and CPCSEA guidelines and no dissection of animals will be done
Course Outcome:
At the end of the course the students will be able to
- gain experience basic designing of immunological tests.
- gain experience in isolation of different cells
- gain experience in handling of animals and its immunization
List of Equipments for 30 Students
- Elisa reader 1
- Microscopes 8
- Microwave oven 1
- Hot plate 4
- Vortex mixer 4
- Table top refrigerated Centrifuge 1
- Fluorescent microsope 1
References:
- Roitt I, Male, Brostoff. Immunology, Mosby Publ., 2002.
- Kuby J, Immunology, WH Freeman and Co., 7th edition 2013
- Ashim K. Chakravarthy, Immunology, TataMcGraw-Hill, 1998.
- Antibodies: A Laboratory Manual, Second edition Edward A. Greenfield, Dana-Farber, Cancer Institute,Cold Spring Harbour Laboratory 2014
- Current protocols in Immunology Volume I-III John E. Coligan (Editor), Ada M. Kruisbeek (Editor), David H. Margulies (Editor), Ethan M. Shevach (Editor), Warren Strober (Editor) 2004
For detailed syllabus of all other subjects of Industrial Technology, 2019 regulation curriculum do visit Industrial Biotech 5th Sem subject syllabuses for 2019 regulation.
For all Industrial Technology results, visit Anna University Industrial Biotech all semester results direct link.