Cell Biology detailed syllabus for Industrial Technology (Industrial Biotech) for 2019 regulation curriculum has been taken from the Anna Universities official website and presented for the Industrial Biotech students. For course code, course name, number of credits for a course and other scheme related information, do visit full semester subjects post given below.
For Industrial Technology 3rd Sem scheme and its subjects, do visit Industrial Biotech 3rd Sem 2019 regulation scheme. The detailed syllabus of cell biology is as follows.
Course Objective:
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Unit I
Biomembranes
Overview of prokaryotic and eukaryotic cells. Lipid bilayer: Composition, structure and properties of membrane lipids. Significance of membrane fluidity and asymmetry. Lipid rafts and its significance. Membrane proteins: Peripheral and various transmembrane proteins with examples -Single pass and multipass transmembrane proteins, Channel proteins and glycosylated proteins. Hydropathy plots. Membrane solubilization and purification: micelle formation. Membrane dynamics: flip-flop, rotational and lateral diffusion. Techniques to assess membrane dynamics: Hybrid cells, FRAP and FLIP techniques. Cell-Cell junctions: Anchoring-, gap- and tight-junctions.
Unit II
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Unit III
Membrane Transport
Basics of membrane transport: Size, solubility and electrochemical gradient of solutes across membrane. Kinetics of passive vs. facilitated diffusion. Transport proteins: Uniporters, Symporters, Antiporters, Aquaporins, ATP driven pumps and its types, Ion-channels – voltage and ligand gated. Role of ion-channels and ATP pumps in nerve conduction. Principles of Patch-Clamp experiment to study ion-channels activity.
Unit IV
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Unit V
Techniques in Cell Biology
Cell fractionation: Extraction, Homogenization and Centrifugation techniques. Microscopy and cell architecture: Fixation, sectioning and staining techniques for microscopic studies – Light microsope, fluorescent/confocal microscope, Electron microscopes – SEM, TEM and cryo EM. Cell isolation: Fluorescence Activated Cell Sorter (FACS) and Magnetic separation/MACS. Cell viability studies: Using tetrazolium salts, LDH release, Tryphan blue exclusion, etc.
Course Outcome:
For the complete syllabus, results, class timetable, and many other features kindly download the iStudy App
It is a lightweight, easy to use, no images, and no pdfs platform to make students’s lives easier..
Text Books:
- Alberts B, Johnson A, Lewis J, Morgan D, Raff M, Roberts K, Walter P. Molecular Biology of the cell, 6th Edition, 2015
- Lodish H, Berk A,Matsudaira P,Kaiser CA, Krieger M, Schot MP, Zipursky L, Darnell J. Molecular Cell Biology, 8th Edition, 2016
- Sadava, D.E. Cell Biology: Organelle Structure and Function, 11th edition 2016
References:
- Cooper, G.M. and R.E. Hansman The Cell: A Molecular Approach, IVth Edition, ASM Press, 2007
- Becker, W.M. etal., The World of the Cell, Vth Edition, Pearson Education, 2003.
- Campbell, N.A., J.B. Recee and E.J. Simon Essential Biology, IIIrd Edition, Pearson International, 2007.
- Alberts, Bruce et al., Essential Cell Biology, IInd Edition, Garland Press (Taylor and Francis), 2004.
For detailed syllabus of all other subjects of Industrial Technology, 2019 regulation curriculum do visit Industrial Biotech 3rd Sem subject syllabuses for 2019 regulation.
For all Industrial Technology results, visit Anna University Industrial Biotech all semester results direct link.