Microbiology Lab detailed syllabus for Industrial Technology (Industrial Biotech) for 2019 regulation curriculum has been taken from the Anna Universities official website and presented for the Industrial Biotech students. For course code, course name, number of credits for a course and other scheme related information, do visit full semester subjects post given below.
For Industrial Technology 2nd Sem scheme and its subjects, do visit Industrial Biotech 2nd Sem 2019 regulation scheme. The detailed syllabus of microbiology lab is as follows.
Course Objective:
The course aims to
- make the student aware of the various lab protocols, the safety measures involved while doing experiments
- equip them in handling microbes confidently.
List of Experiments:
- Introduction, Laboratory Safety, Use of Equipment; Sterilization Techniques
- Culture Media-Types and Use; Preparation of Nutrient broth and agar
- Culture Techniques, Isolation and Preservation of Cultures- Broth: flask, test tubes; Solid: Pour plates, streak plates, slants, stabs
- Microscopy – Working and care of Microscope
- Microscopic Methods in the Study of Microorganisms., Microscopic identification of yeast/mould
- Staining Techniques Simple, Differential- Gram”s Staining
- Quantification of Microbes: Sampling and Serial Dilution; Bacterial count in Soil – TVC
- Effect of Disinfectants- Phenol Coefficient
- Antibiotic Sensitivity Assay
- Growth Curve in Bacteria
- Effect of pH, Temperature, UV radiation on Growth Bacteria
- Biochemical test for identification of E.coli, Bacillus
Equipment Needed for 20 Students
- Autoclave 1
- Hot Air Oven 1
- Incubators 2
- Light Microscopes 4
- Incubator Shaker 1
- Colorimeter 2
- Lamina Flow Chamber 2
- Glassware, Chemicals, Media as required
Course Outcome:
At the end of the course the students will be able to
- identify an organism by microscopic examination, quantification and confirmation by biochemical tests.
- quantify microbes and confirmation of the identity by biochemical tests
- how to use the various equipment in the lab and also the importance of various biosafety measures used in lab
Text Books:
- Cappuccino, J.G. and N. Sherman Microbiology: A Laboratory Manual, 4th Edition, Addison-Wesley, 1999.
- Collee, J.G. etal., Mackie and McCartney Practical Medical Microbiology 4th Edition, Churchill Livingstone, 1996.
For detailed syllabus of all other subjects of Industrial Technology, 2019 regulation curriculum do visit Industrial Biotech 2nd Sem subject syllabuses for 2019 regulation.
For all Industrial Technology results, visit Anna University Industrial Biotech all semester results direct link.