Genetic Engineering Laboratory detail syllabus for Biotechnology (Biotech), 2017 regulation is taken from Anna University official website and presented for students of Anna University. The details of the course are: course code (BT8612), Category (PC), Contact Periods/week (4), Teaching hours/week (0), Practical Hours/week (0). The total course credits are 4.
For all other biotech 6th sem syllabus for b.tech 2017 regulation anna univ you can visit Biotech 6th Sem syllabus for B.Tech 2017 regulation Anna Univ Subjects. The detail syllabus for genetic engineering laboratory is as follows.”
Course Objective:
- Provide hands-on experience in performing basic recombinant DNA techniques.
- Introduce students to the theory behind in each techniques and to describe common applications of each methodology in biological research.
List of Experiments:
- Preparation of plasmid DNA
- Elution of DNA from agarose gels
- Restriction digestion
- Ligation of DNA into expression vectors
- Transformation& Selection of recombinants – Blue white screening assay
- Optimisation of time of inducer for recombinant protein expression
- Expression of protein profiling by SDS – PAGE
- Western blotting, Southern blotting
- PCR amplification of genes
- Colony lysate PCR.
Course Outcome:
By the end of this course, students should be able to:
- Describe the main principles, methods for preparation and cloning of DNA in various organisms.
- Express clearly about the gene amplification and methods for analysis of DNA, such as hybridization, restriction analysis and gene expressions.
- Use genetic and biotechnological techniques to manipulate genetic materials and develops new and improved living organisms.
- Students will be aware of the hazardous chemicals and safety precautions in case of emergency.
Equipment Needed for 30 Students
- Electrphoresis Kit 1
- PCR 1
- Incubators 2
- Light Microscopes 4
- Incubator Shaker 1
- Spectrophotometer 2
- Laminar Flow Chamber 2
- Glassware, Chemicals, Media as required
References:
- Old RW, Primrose SB, Principles Of Gene Manipulation, An Introduction To Genetic Engineering , Blackwell Science Publications, 1993.
- Ansubel FM, Brent R, Kingston RE, Moore DD, Current Protocols In Molecular Biology , Greene Publishing Associates, NY, 1988.
- Berger Sl, Kimmer AR, Methods In Enzymology, Vol 152, Academic Press, 1987
For detail syllabus of all other subjects of B.Tech Biotech, 2017 regulation do visit Biotech 6th Sem syllabus for 2017 Regulation.
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