Genetic Engineering detail syllabus for Biotechnology (Biotech), 2017 regulation is taken from Anna University official website and presented for students of Anna University. The details of the course are: course code (BT8601), Category (PC), Contact Periods/week (4), Teaching hours/week (4), Practical Hours/week (0). The total course credits are given in combined syllabus.
For all other biotech 6th sem syllabus for b.tech 2017 regulation anna univ you can visit Biotech 6th Sem syllabus for B.Tech 2017 regulation Anna Univ Subjects. The detail syllabus for genetic engineering is as follows.”
Course Objective:
- To discuss the gene cloning methods and the tools and techniques involved in gene cloning and genome analysis and genomics.
- To explain the heterologous expression of cloned genes in different hosts.
Unit I
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Unit II
Dna Libraries
Construction of genomic and cDNA libraries, Artificial chromosomes – BACs and YACs, Chromosomal walking, Screening of DNA libraries using nucleic acid probes and antisera.
Unit III
Sequencing and Amplification of Dna
Maxam Gilberts and Sangers methods of DNA sequencing. Inverse PCR, Nested PCR, AFLP-PCR, Allele specific PCR, Assembly PCR, Asymmetric PCR, Hot start PCR, inverse PCR, Colony PCR, single cell PCR, Real-time PCR/qPCR – SYBR green assay, Taqman assay, Molecular beacons. Site directed mutagenesis.
Unit IV
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Unit V
Current Status of Genome Sequencing Projects
Current status of genome sequencing projects, Introduction to Functional genomics, Microarrays, Serial Analysis of Gene expression (SAGE), Subtractive hybridization, DIGE, TOGA, Yeast Two hybrid System, Comparative Genomics, Proteogenomics, Web resources for Genomics, Applications of genome analysis and genomics.
Course Outcome:
- The students after completing this course would be aware of how to clone commercially important genes.
- The students would be aware of how to produce the commercially important recombinant proteins.
- The students would be aware of gene and genome sequencing techniques.
- The students would be aware of microarrays, Analysis of Gene expression and proteomics.
Text Books:
- Old RW, Primrose SB, Principles Of Gene Manipulation, An Introduction To Genetic Engineering , Blackwell Science Publications, 1993.
- Principles of Genome Analysis and Genomics by S.B.Primrose and R.M.Twyman, 3rd Ed. (Blackwell Publishing)
References:
- Ansubel FM, Brent R, Kingston RE, Moore DD, Current Protocols In Molecular BiologyGreene Publishing Associates, NY, 1988.
- Berger Sl, Kimmer AR, Methods In Enzymology, Vol 152, Academic Pres
For detail syllabus of all other subjects of B.Tech Biotech, 2017 regulation do visit Biotech 6th Sem syllabus for 2017 Regulation.
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