Enzyme Technology and Biotransformations detail syllabus for Biotechnology (Biotech), 2017 regulation is taken from Anna University official website and presented for students of Anna University. The details of the course are: course code (BT8403), Category (PC), Contact Periods/week (3), Teaching hours/week (3), Practical Hours/week (0). The total course credits are given in combined syllabus.
For all other biotech 4th sem syllabus for b.tech 2017 regulation anna univ you can visit Biotech 4th Sem syllabus for B.Tech 2017 regulation Anna Univ Subjects. The detail syllabus for enzyme technology and biotransformations is as follows.”
Course Objective:
To enable the students
- To learn enzyme reactions and its characteristics along with the production and purification process
- To give the student a basic knowledge concerning biotransformation reactions with the usage of enzymes
Unit I
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Unit II
Kinetics of Enzyme Action
Kinetics of single substrate reactions; estimation of Michelis – Menten parameters, multisubstrate reactions – mechanisms and kinetics; turnover number; types of inhibition and models -substrate, product. Allosteric regulation of enzymes, Monod Changeux Wyman model, pH and temperature effect on enzymes and deactivation kinetics.
Unit III
Enzyme Immobilization and Biosensors
Physical and chemical techniques for enzyme immobilization – adsorption, matrix entrapment, encapsulation, cross-linking, covalent binding etc., – examples, advantages and disadvantages, design of enzyme electrodes and their application as biosensors in industry, healthcare and environment.
Unit IV
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Unit V
Biotransformation Applications of Enzymes
Hydrolytic- Ester bond, Amide, Epoxides, Nitriles, Reduction reactions -aldehydes, Ketones, C=C, Oxidation reactions – Alkanes, Aromatic, Baeyer-Villiger, Enzymes in organic synthesis – esters, amide, peptide , Modified and Artificial Enzymes , Catalytic antibodies
Course Outcome:
- The knowledge on enzyme and enzyme reactions will be the key step in to proceed towards various concepts in biotechnology.
- The theoretical and practical aspects of kinetics will provide the importance and utility of enzyme kinetics towards research.
- The process of immobilization has been increased steadily in food, pharmaceutical and chemical industries and thus this study will provide simple and easy method of implementation.
- Ideas on Processing, Production and Purification of enzymes at an industrial scale will be helpful to work technologically.
Text Books:
- Trevor Palmer , Enzymes IInd Horwood Publishing Ltd
- Faber K , Biotransformations in Organic Chemistry, IV edition , Springer
References:
- Harvey W. Blanch, Douglas S. Clark, Biochemical Engineering, Marcel Dekker, Inc.
- James M. Lee, Biochemical Engineering, PHI, USA.
- James. E. Bailey and David F. Ollis, Biochemical Engineering Fundamentals, McGraw Hill.
- Wiseman, Enzyme Biotechnology, Ellis Horwood Pub.
For detail syllabus of all other subjects of B.Tech Biotech, 2017 regulation do visit Biotech 4th Sem syllabus for 2017 Regulation.
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