Cell Biology Laboratory detail syllabus for Biotechnology (Biotech), 2017 regulation is taken from Anna University official website and presented for students of Anna University. The details of the course are: course code (BT8311), Category (PC), Contact Periods/week (4), Teaching hours/week (0), Practical Hours/week (0). The total course credits are 4.
For all other biotech 3rd sem syllabus for b.tech 2017 regulation anna univ you can visit Biotech 3rd Sem syllabus for B.Tech 2017 regulation Anna Univ Subjects. The detail syllabus for cell biology laboratory is as follows.”
Course Objective:
- To demonstrate various techniques to learn the morphology, identification and propagation of cells
List of Experiments:
- Introduction to principles of sterile techniques and cell propagation
- Principles of microscopy, phase contrast and fluorescent microscopy
- Identification of given plant, animal and bacterial cells and their components by microscopy
- Grams Staining
- Leishman Staining
- Giemsa Staining
- Thin Layer Chromatography
- Separation of Peripheral Blood Mononuclear Cells from blood
- Osmosis and Tonicity
- Tryphan Blue Assay
- Staining for different stages of mitosis in AlliumCepa (Onion)
Course Outcome:
This practical course will facilitate the students
- To understand the basic techniques to work with cells
- To demonstrate working principles of Microscopy
- To understand and perform cell staining techniques
- To identify the various stages of mitosis
References:
- Rickwood, D. and J.R. Harris Cell Biology: Essential Techniques, Johnwiley, 1996.
- Davis, J.M. Basic Cell Culture: A Practical Approach, IRL, 1994.
Equipment Needed for 30 Students
- Autoclave 1
- Hot Air Oven 1
- Incubators 2
- Light Microscopes 4
- Incubator Shaker 1
- Colorimeter 2
- Lamina Flow Chamber 2
- Glassware, Chemicals, Media as required
For detail syllabus of all other subjects of B.Tech Biotech, 2017 regulation do visit Biotech 3rd Sem syllabus for 2017 Regulation.
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