Genetic Engineering detailed syllabus for Biomedical Engineering (BME) for 2021 regulation curriculum has been taken from the Anna Universities official website and presented for the BME students. For course code, course name, number of credits for a course and other scheme related information, do visit full semester subjects post given below.
For Biomedical Engineering 5th Sem scheme and its subjects, do visit BME 5th Sem 2021 regulation scheme. For Professional Elective-I scheme and its subjects refer to BME Professional Elective-I syllabus scheme. The detailed syllabus of genetic engineering is as follows.
Course Objectives:
Download the iStudy App for all syllabus and other updates.
Unit I
BASICS OF RECOMBINANT DNA TECHNOLOGY
Manipulation of DNA – Restriction and Modification enzymes – Design of linkers and adaptors -Characteristics of cloning and expression vectors – Introduction of recombinant DNA in to host cells and selection methods.
Unit II
DNA LIBRARIES
Construction of genomic and cDNA libraries, Artificial chromosomes – Bacteria, Yeast -Chromosomal walking.
Unit III
Download the iStudy App for all syllabus and other updates.
Unit IV
ORGANIZATION AND STRUCTURE OF GENOMES
Organization and structure of genomes – Genome sequencing methods: Conventional and shotgun genome sequencing methods, Next generation sequencing technologies – Ordering the genome sequence – Genetic maps and Physical maps, STS content based mapping, Hybridization mapping, Optical mapping.
Unit V
CURRENT STATUS OF GENOME SEQUENCING PROJECTS
Introduction to Functional genomics – Microarrays – Serial Analysis of Gene expression (SAGE), Subtractive hybridization, Comparative Genomics, Proteogenomics, Web resources for Genomics, Applications of genome analysis and genomics.
Course Outcomes:
At the end of the course, the student should be able to:
- Would be aware of how to clone commercially important genes.
- The students would be aware of how to produce the commercially important recombinant proteins.
- Will be familiarized with gene and genome sequencing techniques
- Will be aware of microarrays, Analysis of Gene expression and proteomics.
- Acquire ability to function on multi-disciplinary teams
Text Books:
Download the iStudy App for all syllabus and other updates.
Reference Books:
- Isil Aksan Kurnaz, “Techniques in Genetic Engineering”, CRC Press, 2015.
- Oksana Ableitner, “Introduction to Molecular Biology: Working with DNA and RNA (essentials)”, Springer International, 2022.
- Arun K. Shukla, “Proteomics in Biology”, Academic Press, 2017.
For detailed syllabus of all the other subjects of Biomedical Engineering 5th Sem, visit BME 5th Sem subject syllabuses for 2021 regulation.
For all Biomedical Engineering results, visit Anna University BME all semester results direct link.