BT3561: Immunology Laboratory syllabus for BBE 2021 regulation

Immunology Laboratory detailed syllabus for Biotechnology & Biochemical Engineering (BBE) for 2021 regulation curriculum has been taken from the Anna University official website and presented for the BBE students. For course code, course name, number of credits for a course and other scheme related information, do visit full semester subjects post given below.

For Biotechnology & Biochemical Engineering 5th Sem scheme and its subjects, do visit BBE 5th Sem 2021 regulation scheme. The detailed syllabus of immunology laboratory is as follows.

Course Objectives:

• To give practical training in the functioning of immune system.
• To give laboratory training in different immunological and immunotechnological techniques.

Experiments

1. Animal Handling – Immunization – Bleeding techniques by Virtual methods
2. Identification of immune cells in a blood smear and Identification of blood group
3. Isolation of serum and plasma
4. Testing for typhoid antigens by Widal test
5. Immunodiffusion – SRID and Ouchterlony Double Diffusion
6. Immunoelectrophoresis – Classical, Counter Current and Rocket immunoelectrophoresis
7. Enzyme Linked ImmunoSorbent Assay (ELISA) – Types
8. Isolation of peripheral blood mononuclear cells
9. Isolation of monocytes from blood
10. Lymphocyte migration assay
11. Plaque forming cell assay
12. Identification of T cells by T-cell rossetting using sheep RBC.

Course Outcomes:

• The students would be aware of immune system cells and tissues.
• The students would have knowledge on immunological /clinical tests.
• The students would be able to isolate lymphocytes and monocytes.
• The students would be able to identify various immune system cells.
• The students would become familiar with the techniques involved in antigen-antibody reaction
• The students will be able to identify the cellular and molecular basis of immune responsiveness

Reference Books:

1. Roitt I, Male, Brostoff. Immunology, Mosby Publ., 2002.
2. Kuby J, Immunology, WH Freeman & Co., 2000.
3. Ashim K. Chakravarthy, Immunology, TataMcGraw-Hill, 1998.

For detailed syllabus of all other subjects of Biotechnology & Biochemical Engineering, 2021 regulation curriculum do visit BBE 5th Sem subject syllabuses for 2021 regulation.

For all Biotechnology & Biochemical Engineering results, visit Anna University BBE all semester results direct link.