Molecular Biology and Genetic Engineering laboratory detailed syllabus for Biotechnology (BioTech) for 2021 regulation curriculum has been taken from the Anna University official website and presented for the BioTech students. For course code, course name, number of credits for a course and other scheme related information, do visit full semester subjects post given below.
For Biotechnology 5th Sem scheme and its subjects, do visit BioTech 5th Sem 2021 regulation scheme. The detailed syllabus of molecular biology and genetic engineering laboratory is as follows.
Course Objectives:
Download the iStudy App for all syllabus and other updates.
List of Experiments:
- Isolation of total DNA
- Isolation of Plasmid DNA
- Agarose gel electrophoresis
- Restriction enzyme digestion of DNA
- DNA ligation
- Competent cell preparation & Transformation
- Blue-White screening
- Induction and Analysis of Gene expression
- PCR
- SDS-PAGE
- Western blot
Course Outcomes:
Upon completion of the course, students will be able to
- Understand basic techniques of DNA isolation and manipulation.
- Gain experience in selecting genetically transformed organisms for downstream analysis.
- Aware basic techniques involved in analysis of gene expression at nucleic acids and proteins level
- Establish the ability to carry out laboratory experiments and interpret the results.
- Apply practical knowledge to solve biotechnological problems.
- Aware of the hazardous chemicals and safety precautions in case of emergency.
Text Books:
Download the iStudy App for all syllabus and other updates.
Reference Books:
- Keith Wilson and John Walker, “Principles and Techniques of Biochemistry and Molecular Biology” 8th Edition, Cambridge University Press, 2018.
- Alan S. Gerstein, “Molecular Biology Problem Solver-A Laboratory Guide” Wiley-Liss, Inc., 2017.
For detailed syllabus of all other subjects of Biotechnology, 2021 regulation curriculum do visit BioTech 5th Sem subject syllabuses for 2021 regulation.
For all Biotechnology results, visit Anna University BioTech all semester results direct link.