Genetic Engineering detailed syllabus for Biotechnology (BioTech) for 2021 regulation curriculum has been taken from the Anna University official website and presented for the BioTech students. For course code, course name, number of credits for a course and other scheme related information, do visit full semester subjects post given below.
For Biotechnology 5th Sem scheme and its subjects, do visit BioTech 5th Sem 2021 regulation scheme. The detailed syllabus of genetic engineering is as follows.
Course Objectives:
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Unit I
BASICS OF RECOMBINANT DNA TECHNOLOGY 9
Manipulation of DNA – Restriction and Modification enzymes, Design of linkers and adaptors. Characteristics of cloning and expression vectors based on plasmid and bacteriophage, Vectors for insect, yeast and mammalian system, Prokaryotic and eukaryotic host systems, Introduction of recombinant DNA in to host cells and selection methods.
Unit II
DNA LIBRARIES 9
Construction of genomic and cDNA libraries, Artificial chromosomes – BACs and YACs, Chromosomal walking, Screening of DNA libraries using nucleic acid probes and antisera.
Unit III
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Unit IV
ORGANIZATION AND STRUCTURE OF GENOMES 9
Organization and structure of genomes, Genome sequencing methods, Conventional and shotgun genome sequencing methods, Next generation sequencing technologies , Ordering the genome sequence, Genetic maps and Physical maps, STS content based mapping, Restriction Enzyme Finger Printing, Hybridization mapping, Radiation Hybrid Maps, Optical mapping. ORF finding and functional annotation.
Unit V
CURRENT STATUS OF GENOME SEQUENCING PROJECTS 9
Current status of genome sequencing projects, Introduction to Functional genomics, Microarrays, Serial Analysis of Gene expression (SAGE), Subtractive hybridization, DIGE, TOGA, Yeast Two hybrid System, Comparative Genomics, Proteogenomics, Web resources for Genomics, Applications of genome analysis and genomics.
Course Outcomes:
- To clone commercially important genes
- To produce the commercially important recombinant proteins
- To be aware of gene and genome sequencing techniques
- To design modern tools and techniques for manipulation and analysis of genomic sequences
- To develop skills in microarrays, analysis of gene expression and proteomics
- To strategize research methodologies employing genetic engineering techniques
Text Books:
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Reference Books:
- Ansubel FM, Brent R, Kingston RE, Moore DD, “Current Protocols In Molecular Biology“Greene Publishing Associates, NY, 1988.
- Berger Sl, Kimmer AR, “Methods In Enzymology”, Vol 152, Academic Press
For detailed syllabus of all other subjects of Biotechnology, 2021 regulation curriculum do visit BioTech 5th Sem subject syllabuses for 2021 regulation.
For all Biotechnology results, visit Anna University BioTech all semester results direct link.