BT3461: Analytical Instrumentation Laboratory syllabus for BBE 2021 regulation

Analytical Instrumentation Laboratory detailed syllabus for Biotechnology & Biochemical Engineering (BBE) for 2021 regulation curriculum has been taken from the Anna University official website and presented for the BBE students. For course code, course name, number of credits for a course and other scheme related information, do visit full semester subjects post given below.

For Biotechnology & Biochemical Engineering 4th Sem scheme and its subjects, do visit BBE 4th Sem 2021 regulation scheme. The detailed syllabus of analytical instrumentation laboratory is as follows.

Course Objectives:

To train the students

• To have a practical hands on experience on Absoprtion Spectroscopic methods
• To acquire experience in the purification by performing chromatography
• To validate and analysis using spectrometric and microscopic techniques

List of Experiments:

1. Precision and validity in an experiment using absorption spectroscopy 60
2. Validating Lambert-Beers law using KMnO4
3. Finding the molar absorbtivity and stoichiometry of the Fe (1,10 phenanthroline)3 using absorption spectrometry.
4. Finding the pKa of 4-nitrophenol using absorption spectroscopy.
5. UV spectra of nucleic acids.
6. Chemical actinometry using potassium ferrioxolate.
7. Estimation of SO4– by nephelometry.
8. Estimation of Al3+by Flourimetry.
9. Limits of detection using aluminium alizarin complex.
10. Chromatography analysis using TLC.
11. Chromatography analysis using column chromatography.

Equipment Required for a Batch of 30 Students

Colorimeter 2 No. Glassware, Chemicals, Media as required

Course Outcomes:

• The students would visualize and interpret the theory of spectroscopic methods by hands on experiments.

Reference Books:

1. Skoog, D.A. etal. Principles of Instrumental Analysis, Vth Edition, Thomson / Brooks -Cole,1998.
2. Braun, R.D. Introduction to Instrumental Analysis, Pharma Book Syndicate, 1987.
3. Willard, H.H. etal. Instrumental Methods of Analysis, VIth Edition, CBS, 1986.
4. Ewing,G.W. Instrumental Methods of Chemical Analysis, Vth Edition, McGraw-Hill, 1985.

For detailed syllabus of all other subjects of Biotechnology & Biochemical Engineering, 2021 regulation curriculum do visit BBE 4th Sem subject syllabuses for 2021 regulation.

For all Biotechnology & Biochemical Engineering results, visit Anna University BBE all semester results direct link.