Cell and Microbiology Laboratory detailed syllabus for Biotechnology & Biochemical Engineering (BBE) for 2021 regulation curriculum has been taken from the Anna University official website and presented for the BBE students. For course code, course name, number of credits for a course and other scheme related information, do visit full semester subjects post given below.
For Biotechnology & Biochemical Engineering 3rd Sem scheme and its subjects, do visit BBE 3rd Sem 2021 regulation scheme. The detailed syllabus of cell and microbiology laboratory is as follows.
Course Objectives:
- To demonstrate various techniques to learn the morphology, identification and propagation of cells and microbes.
- To learn the staining techniques and culturing of microorganism.
List of Experiments:
- Introduction, Laboratory Safety, Use of Equipment; Sterilization Techniques
- Microscopy – Working and care of Microscope, phase contrast and fluorescent microscopy
- Culture Media-Types and Use; Preparation of Nutrient broth and agar
- Culture Techniques, Isolation and Preservation of Cultures- Broth: flask, test tubes; Solid:Pour plates, streak plates, slants, stabs
- Identification of given plant, animal, bacterial cells and yeast/mould
- Staining Techniques Simple, Differential- Grams Staining, spore /capsule staining, Giemsa, and Leishman Staining
- Quantification of Microbes: Sampling and Serial Dilution; Bacterial count in Soil – TVC
- Effect of Disinfectants- Phenol Coefficient, Antibiotic Sensitivity Assay
- Osmosis and Tonicity and Tryphan Blue Assay
- Growth Curve in Bacteria and Yeast
- Staining for different stages of mitosis in AlliumCepa (Onion)
- Effect of pH, Temperature, UV radiation on Growth Bacteria
Equipment Needed for 30 Students
Autoclave 1 Hot Air Oven 1 Incubators 2 Light Microscopes 4 Incubator Shaker 1 Colorimeter 2 Lamina Flow Chamber 2 Glassware: Petridish, Test tubes,Microscopic slides, Inoculation , loop, Gas burner Chemicals and media Bacterial culture media, Yeast culture media, 70% ethanol ,antibiotics, Crystal violet, Iodine, Safranin, India ink (capsule staining), Immersion oil
Course Outcomes:
Students will be able to
- Understand the advanced technical information pertaining to laboratory bio-safety and preventive measures from pathogenic microorganism.
- Know the various aseptic techniques and sterilization methods.
- Develop the minimum skills to work on several important techniques for the study of microorganisms in the laboratory.
- Learn the various techniques of culturing of microorganisms and media preparation.
- Study the growth of microorganisms by varying the growth conditions.
- Identify the various stages of mitosis
Reference Books:
- Cappuccino, J.G. and N. Sherman Microbiology: A Laboratory Manual, 4th Edition, Addison-Wesley, 1999.
- Collee, J.G. etal., Mackie & McCartney Practical Medical Microbiology 4th Edition, ChurchillLivingstone, 1996Rickwood, D. and J.R. Harris Cell Biology: Essential Techniques, Johnwiley, 1996.
- Davis, J.M. Basic Cell Culture: A Practical Approach, IRL, 1994.
For detailed syllabus of all other subjects of Biotechnology & Biochemical Engineering, 2021 regulation curriculum do visit BBE 3rd Sem subject syllabuses for 2021 regulation.
For all Biotechnology & Biochemical Engineering results, visit Anna University BBE all semester results direct link.