BT3361: Biochemistry Laboratory syllabus for BBE 2021 regulation

Biochemistry Laboratory detailed syllabus for Biotechnology & Biochemical Engineering (BBE) for 2021 regulation curriculum has been taken from the Anna University official website and presented for the BBE students. For course code, course name, number of credits for a course and other scheme related information, do visit full semester subjects post given below.

For Biotechnology & Biochemical Engineering 3rd Sem scheme and its subjects, do visit BBE 3rd Sem 2021 regulation scheme. The detailed syllabus of biochemistry laboratory is as follows.

Aim

• To learn and understand the principles behind the qualitative and quantitative estimation of biomolecules (proteins, carbohydrates, lipids, metabolites etc.,) and laboratory analysis of the same in the body fluids.

List of Experiments:

1. General guidelines for working in biochemistry lab (theory)
2. Units of volume, weight, density and concentration measurements and their range in biological measurements. Demonstration of proper use of volume and weight measurement devices.
3. Accuracy, precision, sensitivity and specificity (theory)
4. Preparation of buffer -titration of a weak acid and a weak base.
5. Qualitative tests for carbohydrates – distinguishing reducing from non-reducing sugars and keto from aldo sugars.
6. Quantitative method for amino acid estimation using ninhydrin – distinguishing amino from imino acid.
7. Protein estimation by Biuret and Lowrys methods.
8. Protein estimation by Bradford and spectroscopic methods.
9. Extraction of lipids and analysis by TLC.
10. Estimation of nucleic acids by absorbance at 260 nm and hyperchromic effect (demo).
11. Enzymatic assay: phosphatase from potato.
12. Enzymatic assay: estimation of glucose by GOD-POD method after hydrolysis of starch with acid and specificity of the enzymatic method.

Equipment Needed for 20 Students

1. Autocalve 1
2. Hot Air Oven 1
3. Incubators 2
4. Light Microscopes 4
5. Incubator Shaker 1
6. Colorimeter 2
7. Laminar Flow Chamber 2
8. Glassware:
9. Test tubes (atleast 10 per student)
10. Beakers – 50 ml, 100 ml, 250 ml one each per student, 500 ml and 1000 ml atleast 5 per batch of 20 students
11. Watch glasses one per student
12. Petridishes as required, glass cuvettes as needed
13. Burette – one per student
14. Glass pipette – one each in 0.5 ml, 1 ml, 5 ml and 10 ml with suitable pipette aid.
15. TLC plate as required for the experiment.
16. Chemicals: glucose, fructose, galactose, maltose, starch, amino acids, DNA, RNA, lipids and commercial enzymes as required. Other chemicals as per the requirement of the standard protocol and commercial kit procured from the vendor followed/ utilised by the deparment

Text Books:

1. Practical Biochemistry by R.C. Gupta and S. Bhargavan.
2. Introduction of Practical Biochemistry by David T. Phummer. (II Edition)

Reference Books:

1. Harpers Biochemistry Ed. R.K. Murray , D.K. Granner, P.A. Mayes and V.W.Rodwell, Appleton and Lange ,Stanford ,Conneticut.
2. Textbook of Biochemistry with clinical correlations. Ed. Thomas M. Devlin. Wiley Liss Publishers

For detailed syllabus of all other subjects of Biotechnology & Biochemical Engineering, 2021 regulation curriculum do visit BBE 3rd Sem subject syllabuses for 2021 regulation.

For all Biotechnology & Biochemical Engineering results, visit Anna University BBE all semester results direct link.