Modern Bio Analytical Techniques detailed syllabus for Biotechnology (BioTech) for 2021 regulation curriculum has been taken from the Anna Universities official website and presented for the BioTech students. For course code, course name, number of credits for a course and other scheme related information, do visit full semester subjects post given below.
For Biotechnology 5th Sem scheme and its subjects, do visit BioTech 5th Sem 2021 regulation scheme. For Professional Elective-II scheme and its subjects refer to BioTech Professional Elective-II syllabus scheme. The detailed syllabus of modern bio analytical techniques is as follows.
Course Objectives:
Download the iStudy App for all syllabus and other updates.
Unit I
SPECTROSCOPY STUDY OF CHEMICAL COMPOUNDS AND BIO-MOLECULES 9
Electromagnetic radiations and interactions with matters: Electromagnetic spectrum. Quantisation of energy, Electronic, vibrational and rotational spectroscopy. Franck-Condon principle, Jablonski diagram, radiative, nonradiative pathways, fluorescence and phosphorescence. Absorption of radiation, BeerLambert’s law, deviation of Beer-Lambert’s equation and its limitations. Principals, instrumentation, sampling and application of few spectroscopic techniques: UV-Visible spectroscopy, Fluorescence spectroscopy, IR/Raman spectroscopy, NMR Spectroscopy and Mass spectroscopy.
Unit II
DIFFRACTION TECHNIQUE 9
Introduction to lattice and lattice systems, Bragg’s plane, miller indices, point groups and space groups Principle of diffraction and X-ray diffraction: X-rays production, X- ray spectra, Bragg’s law and intensity of X- rays, Mosley’s law, powdered XRD, percentage crystallinity, single crystal XRD, macromolecular XRD (protein crystallization, data collection and structure solution).
Unit III
Download the iStudy App for all syllabus and other updates.
Unit IV
MICROSCOPY 9
Microscopy with light and electrons – Electrons and their interaction with the specimen – Electron, diffraction – Instrument, specimen preparation and application of TEM and SEM – Fluorescence microscopy – Laser confocal microscopy – Phase contrast – Video microscopy – Scanning probe microscopy.
Unit V
ELECTROPHORETIC TECHNIQUES 9
Principle, equipment and process, Agarose gel electrophoresis, horizontal and vertical gel electrophoresis, electrophoresis techniques, Isoelectric focusing, capillary electrophoresis and application of electrophoresis in analysing macromolecules.
Course Outcomes:
- The students will be capable of handling different instruments in the laboratory.
- They would be able to compare different separation techniques and use them effectively in research work
Text Books:
Download the iStudy App for all syllabus and other updates.
Reference Books:
- R. F. Boyer, Modern experimental biochemistry (Benjamin Cummings, San Francisco, 3rd ed., 2000).
- J. R. Lakowicz, Principles of fluorescence spectroscopy (Springer, New York, 2006;
- B. Fultz, Transmission electron microscopy and diffractometry of materials (Springer, Berlin ; New York, 2nd ed., 2002).
- D. B. Williams, C. B. Carter, Transmission electron microscopy a textbook for materials science (Springer, New York, 2009
- R. M. Silverstein, Spectrometric identification of organic compounds (John Wiley & Sons, Hoboken, NJ, 7th ed., 2005).
- D. Harvey, Modern analytical chemistry (McGraw-Hill, Boston, 2000).
- Pavia, D.L., Lampman, G.M., Kriz, G.S. and Vyvyan, J.R., “Introduction to Spectroscopy”, 4th Edition, Brooks/Cole Cengage Learning, 2008.
For detailed syllabus of all the other subjects of Biotechnology 5th Sem, visit BioTech 5th Sem subject syllabuses for 2021 regulation.
For all Biotechnology results, visit Anna University BioTech all semester results direct link.