Protein Engineering detailed syllabus for Biotechnology (BioTech) for 2021 regulation curriculum has been taken from the Anna Universities official website and presented for the BioTech students. For course code, course name, number of credits for a course and other scheme related information, do visit full semester subjects post given below.
For Biotechnology 5th Sem scheme and its subjects, do visit BioTech 5th Sem 2021 regulation scheme. For Professional Elective-II scheme and its subjects refer to BioTech Professional Elective-II syllabus scheme. The detailed syllabus of protein engineering is as follows.
Course Objectives:
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Unit I
BONDS, ENERGIES, BUILDING BLOCKS OF PROTEINS 9
Covalent, Ionic, Hydrogen, Coordinate, hydrophobic and Vander walls interactions in protein structure. Interaction with electromagnetic radiation (radio, micro, infrared, visible, ultraviolet, X-ray) and elucidation of protein structure. Amino acids (the students should be thorough with three and single letter codes) and their molecular properties (size, solubility, charge, pKa), Chemical reactivity in relation to post-translational modification (involving amino, carboxyl, hydroxyl, thiol, imidazole groups).
Unit II
PROTEIN ARCHITECTURE 9
Primary structure: peptide mapping, peptide sequencing – automated Edman method & massspec. High-throughput protein sequencing setup Secondary structure: Alpha, beta and loop structures and methods to determine Super-secondary structure: Alpha-turn-alpha, beta-turnbeta (hairpin), beta-sheets, alpha-beta-alpha, topology diagrams, up and down & TIM barrel structures nucleotide binding folds, prediction of substrate binding sites.
Unit III
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Unit IV
STRUCTURE-FUNCTION RELATIONSHIP 9
DNA-binding proteins: prokaryotic transcription factors, Helix-turn-Helix motif in DNA binding, Trp Repressor, Eukaryotic transcription factors, Zn fingers, helix-turn helix motifs in homeodomain, Leucine zippers. Membrane proteins: General characteristics, Transmembrane segments, prediction, bacteriorhodopsin and Photosynthetic reaction center, Immunoglobulins: IgG Light chain and heavy chain architecture, abzymes and Enzymes: Serine proteases, understanding catalytic design by engineering trypsin, chymotrypsin and elastase, substrate-assisted catalysis other commercial applications. Computer exercise on the above aspects
Unit V
PROTEOMICS 9
Introduction to the concept of proteome, components of proteomics, proteomic analysis, importance of proteomics in biological functions, protein-protein interactions and methods to study it: protein arrays, cross linking methods, affinity methods, yeast hybrid systems and protein arrays. Computer exercise on the above aspects
Course Outcomes:
Upon completion of this course the student will be able to
- Analyze the various interactions in protein makeup.
- Be familiar with different levels of protein structure.
- Know the role of functional proteins in various field of study.
- Practice the latest application of protein science in their research.
- Understand the major factors for protein folding.
- Analyze and compare protein sequence data.
Text Books:
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Reference Books:
- Voet D. and Voet G., “Biochemistry”. 3rd Edition. John Wiley and Sons, 2008.
- Haggerty, Lauren M.“Protein Structure: Protein Science and Engineering”.Nova Science Publications, 2011.
- Williamson, Mike “How Proteins Work”. Garland Science, 2012.
For detailed syllabus of all the other subjects of Biotechnology 5th Sem, visit BioTech 5th Sem subject syllabuses for 2021 regulation.
For all Biotechnology results, visit Anna University BioTech all semester results direct link.