Bioreactor Considerations for Recombinant Products detailed syllabus for Biotechnology (BioTech) for 2021 regulation curriculum has been taken from the Anna Universities official website and presented for the BioTech students. For course code, course name, number of credits for a course and other scheme related information, do visit full semester subjects post given below.
For Biotechnology 5th Sem scheme and its subjects, do visit BioTech 5th Sem 2021 regulation scheme. For Professional Elective-I scheme and its subjects refer to BioTech Professional Elective-I syllabus scheme. The detailed syllabus of bioreactor considerations for recombinant products is as follows.
Course Objectives:
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Unit I
GENETICALLY ENGINEERED ORGANISMS 9
Different host vector systems, Guidelines for choosing Host Vector systems, Process constraints -Genetic instability, considerations in plasmid design, Regulatory constraints, principles and implementation of containment, good industrial large-scale practice (GILSP).
Unit II
CONSIDERATIONS FOR ANIMAL CELL CULTURES 9
Structure and biochemistry of animal cells – Methods Used for the cultivation of animal cells -Bioreactor considerations for animal cell culture – Products of animal cell cultures, economics of animal cell tissue cultures.
Unit III
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Unit IV
DOWNSTREAM PROCESSING CONSIDERATIONS 9
Release of protein from Biological Host, genetic approaches to facilitate protein purification, SolidLiquid separation, extraction of Recombinant protein, Avoidance of proteolysis from extracts, membranes for protein isolation and purification, Chromatographic techniques, Removal of detergent from protein fractions, precipitation of proteins, protein crystallization for large scale bio separation.
Unit V
SAFETY CONSIDERATIONS ASSOCIATED WITH AGRICULTURAL AND
ENVIRONMENTAL APLLICATIONS 9
Risk assessment methods, safety considerations, Application of rDNA organism in the environment, Survival, multiplication and/or dissemination in the environment, Interactions with species or biological systems, effects on the environment, evaluating environmental risks of rDNA organisms released from industrial applications.
Course Outcomes:
Upon completion of this course the student will be able to
- To acquire skills on techniques of isolation of gene of interest and construction of recombinant DNA.
- To apply techniques for production of pharmaceuticals, growth hormones, vaccines, gene therapy in expression system.
- To apply rDNA technology in evolving plants for resistance to pest and disease, tolerance to herbicides and abiotic factors.
- To identify problems associated with production of recombinant proteins and protein purification and devising strategies to overcome problem.
- To acquire knowledge on environmental applications of genetic engineering through bioremediation.
- To identify the methods for selection of recombinants and to express recombinant protein in E. coli and eukaryotes.
Text Books:
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Reference Books:
- Portner, R. and Barradas, O.B.J.P., 2007. Animal cell biotechnology. Methods and Protocols, 2nd. Edition. Humana.
- Slater, A., Scott, N. and Fowler, M., 2008. Plant biotechnology: the genetic manipulation of plants. OUP Oxford.
- Portner, R. and Barradas, O.B.J.P., 2007. Animal cell biotechnology. Methods and Protocols, 2nd. Edition. Humana.
- Slater, A., Scott, N. and Fowler, M., 2008. Plant biotechnology: the genetic manipulation of plants. OUP Oxford.
For detailed syllabus of all the other subjects of Biotechnology 5th Sem, visit BioTech 5th Sem subject syllabuses for 2021 regulation.
For all Biotechnology results, visit Anna University BioTech all semester results direct link.