Bioprocess Laboratory I detail syllabus for Biotechnology (Biotech), 2017 regulation is taken from Anna University official website and presented for students of Anna University. The details of the course are: course code (BT8511), Category (PC), Contact Periods/week (4), Teaching hours/week (0), Practical Hours/week (0). The total course credits are 4.
For all other biotech 5th sem syllabus for b.tech 2017 regulation anna univ you can visit Biotech 5th Sem syllabus for B.Tech 2017 regulation Anna Univ Subjects. The detail syllabus for bioprocess laboratory i is as follows.”
Course Objective:
- To train the students on enzyme characterization, immobilization and medium optimization methods.
- To train on methods to investigate the growth of microorganisms in different systems under different conditions.
List of Experiments:
- Enzyme kinetics – Determination of Michaelis – Menten parameters
- Enzyme activity – Effect of Temperature and Deactivation Kinetics
- Enzyme activity – Effect of pH
- Enzyme inhibition kinetics
- Enzyme immobilization – Gel entrapment
- Enzyme immobilization -Cross-linking
- Enzymatic conversion in Packed bed Column
- Growth of Bacteria – Estimation of Biomass, Calculation of Specific Growth Rate, Yield Coefficient
- Optimization by Plackett Burman Design
- Optimization by Response Surface Methodology
Course Outcome:
At the end of this course, students will be able to:
- Explain about Enzyme kinetics and characterization and how to use them for practical applications.
- Evaluate the growth kinetics of microorganisms and become adept with medium optimization techniques.
- Determine an experimental objective, understand the theory behind the experiment, and operate the relevant equipment safely.
- Demonstrate good lab citizenry and the ability to work in team.
Equipment Needed for 20 Students
Autocalve 1
Hot Air Oven 1
Incubators 2
Light Microscopes 4
Incubator Shaker 1
Colorimeter 2
Laminar Flow Chamber 2
Glassware, Chemicals, Media as required
References:
- Bailey and Ollis, Biochemical Engineering Fundamentals, McGraw Hill (2nd Ed.), 1986.
- Shuler and Kargi, Bioprocess Engineering , Prentice Hall, 1992.
- Pauline Doran, Bioprocess Engineering Calculation, Blackwell Scientific Publications.
- Peter F. Stanbury, Stephen J. Hall and A. Whitaker, Principles of Fermentation Technology,
- Science and Technology Books.
For detail syllabus of all other subjects of B.Tech Biotech, 2017 regulation do visit Biotech 5th Sem syllabus for 2017 Regulation.
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