Bio Chemistry Laboratory detail syllabus for Biomedical Engineering (Bme), 2017 regulation is taken from Anna University official website and presented for students of Anna University. The details of the course are: course code (BM8211), Category (PC), Contact Periods/week (4), Teaching hours/week (0), Practical Hours/week (0). The total course credits are 4.
For all other bme 2nd sem syllabus for be 2017 regulation anna univ you can visit Bme 2nd Sem syllabus for BE 2017 regulation Anna Univ Subjects. The detail syllabus for bio chemistry laboratory is as follows.”
Course Objective:
- To provide practice on:
- Estimation and quantification of biomolecules.
- Separation of macromolecules.
- Estimation and interpretation of biochemical parameter.
List of Experiments:
- General guidelines for working and functional component of biochemistry lab
- Preparation of solutions: 1) percentage solutions, 2) molar solutions, 3) normal solutions
- Standardization of pH meter, preparation of buffers, emulsions.
- Spectroscopy: Determination of absorption maxima (Amax) of a given solution
- General tests for carbohydrates, proteins and lipids.
- Identification of Blood Collection Tubes and Phlebotomy equipments
- Preparation of serum and plasma from blood.
- Estimation of Haemoglobin
- Estimation of blood glucose.
- Estimation of creatinine.
- Estimation of urea.
- Estimation of Uric acid
- Estimation of cholesterol
- Assay of SGOT/SGPT.
- ELISA test
- Separation of proteins by SDS electrophoresis(Demo)
- Separation of amino acids by thin layer chromatography (Demo).
Course Outcome:
Upon completion of the course, students will be able to:
- Understand the Biochemistry laboratory functional components
- Understand the basics principle of preparation of buffers.
- Have a sound knowledge of qualitative test of different biomolecules.
- Understand the basics knowledge of Biochemical parameter and their interpretation in Blood sample.
- Have a sound knowledge of separation technology of proteins and aminoacids.
Lab Requirement for a Batch of 30 Students:
Requirement for a batch of 30 students
- Colorimeter 2 Nos
- Spectrophotometer 1 No.
- pH meter 1 No
- Weighing balance 1 No
- Refrigerator 1 No
- SDS gel electrophoresis 1 No
- TLC, ready TLC plates 1 No Wintrobes tube 2 Nos.
- Centrifuge Normal 1 No
- Microslides 2 packets
- Lancet 5 boxes
- Microscope 1 No
- Neubaurs Chamber 2 Nos.
- Heparinized Syringe 1box
- Haemoglobinometer 1 No
- Elisa reader.1 nos
- Capillary tubes 1 box
For detail syllabus of all other subjects of BE Bme, 2017 regulation do visit Bme 2nd Sem syllabus for 2017 Regulation.
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