Metabolic Engineering detailed syllabus for Biotechnology & Biochemical Engineering (BBE) for 2021 regulation curriculum has been taken from the Anna Universities official website and presented for the BBE students. For course code, course name, number of credits for a course and other scheme related information, do visit full semester subjects post given below.
For Biotechnology & Biochemical Engineering 5th Sem scheme and its subjects, do visit BBE 5th Sem 2021 regulation scheme. For Professional Elective-II scheme and its subjects refer to BBE Professional Elective-II syllabus scheme. The detailed syllabus of metabolic engineering is as follows.
Course Objectives:
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Unit I
CELL METABOLIC ENGINEERING, METABOLIC PATHWAYS 9
Improvement of cellular properties, altering transport of nutrients including carbon and nitrogen; Methods for metabolic characterization: Genome, Transcriptome, Proteome. Feedback control systems, alteration of feedback regulation for enhanced production of primary metabolites: glutamic acid, Mutants which do not produce feedback inhibitors or repressors- Auxotrophslysine, purine nucleotides; trophophase- idiophase relationship; secondary metabolites- Antibiotics, Mycotoxins
Unit II
MANIPULATION OF METABOLIC PATHWAYS 9
Pathway manipulation strategies for overproduction of various metabolites, examples of ethanol overproduction, overproduction of intermediates in main glycolytic pathway and TCA cycle like pyruvate, succinate; Tools for multiple genomic modifications examples- TALENS CRISPR-Cas systems as well as traditional systems of gene knock ins and knock outs and promoter engineering.
Unit III
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Unit IV
BASICS OF SYNTHETIC BIOLOGY 9
Synthetic biology – definitions and concepts. History and evolution of synthetic biology and engineering perspectives. Natural vs Engineering systems. Tools of synthetic biology -Key enabling technologies in synthetic biology. BioBricks – Definition of a BioBrick
Unit V
APPLICATIONS OF METABOLIC ENGINEERING AND SYNTHETIC BIOLOGY 9
Product over production examples: amino acids, By-product minimization of acetate in recombinant E. coli, Extension of substrate utilization range for organisms such as S. cerevisae for ethanol production; Application of synthetic biology with a case study.
Course Outcomes:
- Comprehend modern biology with engineering principles
- Recall the basic principles and regulation of metabolic pathways
- Construct suitable metabolic flux models using available metabolic engineering tools
- Identify the appropriate host and/or metabolic pathways to produce a desired product
- Compare the potential metabolic engineering strategies using quantitative metabolic modelling
- Apply the concept of synthetic biology in interdisciplinary research
Text Books:
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Reference Books:
- Freemont, P.S and Kitney, R.I. (2012). Synthetic Biology – a Primer. World Scientific Publishing Co pvt Ltd
- Peter F. Stanbury, Stephen J. Hall & A. Whitaker, “Principles of Fermentation Technology”, Butterworth – Heinemann An Imprint of Elsevier India Pvt. Ltd., 3rd edition, 2016
- Crueger W. and Crueger A., “A Text Book of Industrial Microbiology”, Panima Publishing Corporation, 2005
- Cheng Q. “Microbial Metabolic Engineering: Methods and Protocols”, Humana Press, First Edition (2011).
- Lehninger, A. L, Nelson D. L and Cox, M. M, “Principles of Biochemistry”, Freeman Publishers, New York, Seventh edition, 2017
For detailed syllabus of all the other subjects of Biotechnology & Biochemical Engineering 5th Sem, visit BBE 5th Sem subject syllabuses for 2021 regulation.
For all Biotechnology & Biochemical Engineering results, visit Anna University BBE all semester results direct link.