Downstream processing Lab Syllabus for B.Tech Seventh sem is covered here. This gives the details about credits, number of hours and other details along with reference books for the course.
The detailed syllabus for Downstream processing Lab B.Tech 2016-2017 (R13) seventhsem is as follows
OBJECTIVES:
To provide hands on training in Down stream processing through simple experimentations in the laboratory. This will be a pre-requisite for project work.
The objectives of this course is to practice the students
- To understand the nature of the end product, its concentration, stability and degree of purification required
- To design processes for the recovery and subsequent purification of target biological products.
LIST OF EXPERIMENTS
TOTAL : 60 PERIODS
OUTCOMES: Upon success completion of this course, the students would have
- Acquired knowledge for the separation of whole cells and other insoluble ingradients from the culture broth.
- Learned cell disruption techniques to release intracellular products
- Learned various techniques like evaporation, extraction, precipitation, membrane separation for concentrating biological products
- Learned the basic principles and techniques of chromatography to purify the
biological products and formulate the products for different end uses.
LIST OF EQUIPMENT FOR BATCH OF 30 STUDENTS
- Centrifuge 1
- Cross flow filtration set up 2
- FPLC 1
- Sonicator 1
- French press 1
REFERENCES:
- P.A. Belter, E.L. Cussler And Wei-Houhu – Bioseparations – Downstream Processing For Biotechnology, Wiley Interscience Pun. (1988).
- R.O. Jenkins, (Ed.) – Product Recovery In Bioprocess Technology – Biotechnology By Open Learning Series, Butterworth-Heinemann (1992).
- J.C. Janson And L. Ryden, (Ed.) – Protein Purification – Principles, High Resolution
Methods And Applications, VCH Pub. 1989.
For all other B.Tech BT 7th sem syllabus go to Anna University B.Tech BIOTECHNOLOGY 7th Sem Course Structure for (R13) Batch. All details and yearly new syllabus will be updated here time to time. Subscribe, like us on facebook and follow us on google plus for all updates.
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