Downstream processing Based for Biotechnologists Syllabus for B.Tech Seventh sem is covered here. This gives the details about credits, number of hours and other details along with reference books for the course.
The detailed syllabus for Downstream processing B.Tech 2016-2017 (R13) seventhsem is as follows
OBJECTIVES: To enable the students to
- Understand the methods to obtain pure proteins, enzymes and in general about
product development R & D - Have depth knowledge and hands on experience with on Downstream processes
UNIT I : DOWNSTREAM PROCESSING [8 Hours]
Introduction to downstream processing principles characteristics of biomolecules and bioprocesses. Cell disruption for product release – mechanical, enzymatic and chemical methods. Pretreatment and stabilisation of bioproducts.
UNIT II : PHYSICAL METHODS OF SEPERATION [8 Hours]
Unit operations for solid-liquid separation – filtration and centrifugation.
UNIT III : ISOLATION OF PRODUCTS [10 Hours]
Adsorption, liquid-liquid extraction, aqueous two-phase extraction, membrane separation – ultrafiltration and reverse osmosis, dialysis, precipitation of proteins by different methods.
TOTAL : 45 PERIODS
OUTCOMES: Upon success completion of this course, the students will be able to:
- Define the fundamentals of downstream processing for product recovery
- Understand the requirements for successful operations of downstream processing
- Describe the components of downstream equipment and explain the purpose of each
- Apply principles of various unit operations used in downstream processing and
enhance problem solving techniques required in multi-factorial manufacturing environment in a structured and logical fashion.
TEXTBOOKS:
- Belter, P.A. E.L. Cussler And Wei-Houhu – “Bioseparations – Downstream Processing For Biotechnology, Wiley Interscience Pun. (1988).
- Sivasankar, B. “Bioseparations : Principles and Techniques”. PHI, 2005.
REFERENCES:
- R.O. Jenkins, (Ed.) – Product Recovery In Bioprocess Technology – Biotechnology By Open Learning Series, Butterworth-Heinemann (1992).
- J.C. Janson And L. Ryden, (Ed.) – Protein Purification – Principles, High Resolution
Methods And Applications, VCH Pub. 1989. - R.K. Scopes – Protein Purification – Principles And Practice, Narosa Pub. (1994).
For all other B.Tech BT 7th sem syllabus go to Anna University B.Tech BIOTECHNOLOGY 7th Sem Course Structure for (R13) Batch. All details and yearly new syllabus will be updated here time to time. Subscribe, like us on facebook and follow us on google plus for all updates.
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