Metabolic Engineering for Biotechnologists Syllabus for B.Tech Sixth sem is covered here. This gives the details about credits, number of hours and other details along with reference books for the course.
The detailed syllabus for Metabolic Engineering B.Tech 2016-2017 (R13) sixthsem is as follows
OBJECTIVES:
- To provide a quantitative basis, based on thermodynamics, enzyme kinetics, for the understanding of metabolic networks in single cells and at the organ level.
- To enable the students to use organisms to produce valuable substances on an
industrial scale in cost effective manner.
UNIT I : SUCCESSFUL EXAMPLES OF METABOLIC ENGINEERING [9 Hours]
Product over production examples: amino acids, polyhydroxyalkanoic acids, By-product minimization of acetate in recombinant E. coli, Extension of substrate utilization range for organisms such as S. cerevisae and Z. mobilis for ethanol production, Improvement of cellular properties, Altering transport of nutrients including carbon and nitrogen and xenobiotic degradation.
UNIT II : METABOLIC FLUX ANALYSIS [9 Hours]
Comprehensive models of cellular reactions; stoichiometry of cellular reactions, reaction rates, dynamic mass balances, metabolic flux analysis. MFA of exactly determined systems, over determined systems.
UNIT III : CONSTRAINT BASED GENOMIC SCALE METABOLIC
MODEL [9 Hours]
Underdetermined systems- linear programming, sensitivity analysis, Development of Genomic scale metabolic model, Flux balance analysis, Regulatory on-off Minimization and Minimization of metabolic adjustments and Opt knock tool development, Elementary mode analysis, Extreme pathways.
TOTAL: 45 PERIODS
OUTCOMES: After completion of metabolic engineering, students will be able
- To learn stoichiometry and energetics of metabolism.
- To apply practical applications of metabolic engineering in chemical,energy,medical
- and environmental fields.
- To integrate modern biology with engineering principles.
- To design a system, component, or process to meet desired needs.
TEXT BOOKS:
- Stephanopoulos, G.N. “Metabolic Engineering : Principles and Methodologies”.
Academic Press / Elsevier, 1998. - Lee, S.Y. and Papoutsakis, E.T. “Metabolic Engineering”. Marcel Dekker, 1998.
- Nielsen, J. and Villadsen, J. “Bioreaction Engineering Principles”. Springer, 2007.
REFERENCES:
- Voit, E.O. “Computational Analysis of Biochemical Systems : A Practical Guide for
Biochemists and Molecular Biologists”. Cambridge University Press, 2000. - Scheper, T. “Metabolic Engineering” Vol 73 (Advances in Biochemical Engineering
Biotechnology) Springer, 2001. - S. Cortassa, M.A.Aon,A.A.Iglesias and D.Llyod, “ An Introduction to Metabolic and
Cellular Engineering”, World Scientific Publishing Co. Pte. Ltd, 2002. - Christiana D. Smolke, “ The Metabolic Pathway Engineering Handbook Fundamentals”, CRC Press Taylor & Francis Group, 2010.
- Boris N. Kholodenko and Hans V. Westerhoff “Metabolic Engineering in the Post
Genomic Era”, Horizon Bioscience, 2004.
For all other B.Tech BT 6th sem syllabus go to Anna University B.Tech BIOTECHNOLOGY 6th Sem Course Structure for (R13) Batch. All details and yearly new syllabus will be updated here time to time. Subscribe, like us on facebook and follow us on google plus for all updates.
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