Genetic Engineering and Genomics for Biotechnologists Syllabus for B.Tech Sixth sem is covered here. This gives the details about credits, number of hours and other details along with reference books for the course.
The detailed syllabus for Total Quality Management for Genetic Engineering and Genomics B.Tech 2016-2017 (R13) sixthsem is as follows
OBJECTIVES:
- To discuss the gene cloning methods and the tools and techniques involved in gene cloning and genome analysis and genomics.
- To explain the heterologous expression of cloned genes in different hosts,
production of recombinant proteins and PCR techniques. - To explain comparative genomics and proteomics.
UNIT I : BASICS OF RECOMBINANT DNA TECHNOLOGY [9 Hours]
Manipulation of DNA – Restriction and Modification enzymes, Design of linkers and adaptors. Characteristics of cloning and expression vectors based on plasmid and bacteriophage, Vectors for yeast, insect and mammalian systems, Prokaryotic and eukaryotic expression host systems, Introduction of recombinant DNA in to host cells and selection methods.
UNIT II : DNA LIBRARIES [9 Hours]
Construction of genomic and cDNA libraries, Artificial chromosomes – BACs and
YACs, Chromosome walking, Screening of DNA libraries using nucleic acid probes and antisera.
UNIT III : SEQUENCING AND AMPLIFICATION OF DNA [9 Hours]
Maxam Gilbert’s and Sanger Coulson’s and automated methods of DNA sequencing, Inverse PCR, Nested PCR, AFLP-PCR, Allele specific PCR, Assembly PCR, Asymmetric PCR, Hot start PCR, Colony PCR, single cell PCR, Real-time PCR/qPCR – SYBR green assay, Taqman assay, Molecular beacons, Site directed mutagenesis.
TOTAL (L:45 + T:15) : 60 PERIODS
OUTCOMES:
- The students after completing this course would be aware of how to clone commercially important genes. The students would be aware of how to produce the commercially important recombinant proteins.
- The students would be aware of gene and genome sequencing techniques.
- The students would be aware of microarrays, Analysis of Gene expression and
proteomics.
TEXT BOOKS:
- Primrose SB and R. Twyman “Principles Of Gene Manipulation & Geneomics
Blackwell Science Publications, 2006. - Principles of Genome Analysis and Genomics by S.B.Primrose and R.M.Twyman, Third Edition (Blackwell Publishing), 2003.
REFERENCES:
- Ansubel FM, Brent R, Kingston RE, Moore DD, “Current Protocols In Molecular Biology “Greene Publishing Associates, NY, 1988.
- Berger Sl, Kimmer AR, “Methods In Enzymology”, Vol 152, Academic Press, 1987.
- Genomes 3 by T.A.Brown, Third Edition (Garland Science Publishing), 2007.
For all other B.Tech BT 6th sem syllabus go to Anna University B.Tech BIOTECHNOLOGY 6th Sem Course Structure for (R13) Batch. All details and yearly new syllabus will be updated here time to time. Subscribe, like us on facebook and follow us on google plus for all updates.
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