Protein Structure Function and Proteomics Syllabus for B.Tech Fifth sem is covered here. This gives the details about credits, number of hours and other details along with reference books for the course.
The detailed syllabus for Protein Structure Function and Proteomics B.Tech 2016-2017 (R13) fifthsem is as follows
OBJECTIVES: To enable the students
- To identify the importance of protein biomolecules.
- To realize the structure-function relationships in proteins.
UNIT I : BONDS, ENERGIES, BUILDING BLOCKS OF PROTEINS [9 Hours]
Covalent, Ionic, Hydrogen, Coordinate, hydrophobic and Vander walls interactions in protein structure. Interaction with electromagnetic radiation (radio, micro, infrared, visible, ultraviolet, X-ray) and elucidation of protein structure. Amino acids (the students should be thorough with three and single letter codes) and their molecular properties (size, solubility, charge, pKa), Chemical reactivity in relation to post-translational modification (involving amino, carboxyl, hydroxyl, thiol, imidazole groups).
UNIT II : PROTEIN ARCHITECTURE [9 Hours]
Primary structure: peptide mapping, peptide sequencing – automated Edman method & mass- spec. High-throughput protein sequencing setup Secondary structure: Alpha, beta and loop structures and methods to determine Super-secondary structure: Alpha-turnalpha, beta-turn- beta (hairpin), beta-sheets, alpha-beta-alpha, topology diagrams, up and down & TIM barrel structures nucleotide binding folds.
UNIT III: TERTIARY STRUCTURE [9 Hours]
Prediction of substrate binding sites, Tertiary structure: Domains, folding, denaturation and renaturation, overview of methods to determine 3D structures. Quaternary structure: Modular nature, formation of complexes, protein-protein interactions and methods to study it: Computer exercise on the above aspects
[TOTAL (L:45 + T:15) : 60 PERIODS]
OUTCOMES:
Upon completion of this course, students will be able:
- To analyze the various interactions in protein makeup.
- To be familiar with different levels of protein structure.
- To know the role of functional proteins in various field of study.
- To practice the latest application of protein science in their research.
TEXT BOOKS:
- Branden C. and Tooze J., “Introduction to Protein Structured”2nd Edition, Garlan Publishing, 1999.
- Creighton T.E. “Proteins” 2nd Edition. W.H. Freeman, 1993.
- Pennington, S.R and M.J. Dunn, “Proteomics : Protein Sequence to Function”. Viva Books, 2002
REFERENCE:
- Liebler, “Introduction to Proteomics” Humana Press, 2002.
For all other B.Tech BT 5th sem syllabus go to Anna University B.Tech BIOTECHNOLOGY 5th Sem Course Structure for (R13) Batch. All details and yearly new syllabus will be updated here time to time. Subscribe, like us on facebook and follow us on google plus for all updates.
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