Enzyme Technology and Biotransformation Syllabus for B.Tech Fourth sem is covered here. This gives the details about credits, number of hours and other details along with reference books for the course.
The detailed syllabus for Enzyme Technology and Biotransformation B.Tech 2016-2017 (R13) fourthsem is as follows
OBJECTIVES: To enable the students
- To learn enzyme reactions and its characteristics along with the production and purification process
- To give the student a basic knowledge concerning biotransformation reactions with the usage of enzymes
UNIT I : INTRODUCTION TO ENZYMES [9 Hours]
Classification of enzymes – Mechanisms of enzyme action – Concept of active site and energetics of enzyme substrate complex formation – Specificity of enzyme action – Principles of catalysis – Collision theory and transition state theory – Role of entropy in catalysis.
UNIT II : KINETICS OF ENZYME ACTION [9 Hours]
Kinetics of single substrate reactions; estimation of Michelis-Menten parameters – Multisubstrate reactions – Mechanisms and kinetics – Turnover number – Types of inhibition and models for substrate and product – Allosteric regulation of enzyme – Monod Changeux Wyman model – pH and temperature effect on enzymes & deactivation kinetics.
UNIT III : ENZYME IMMOBILIZATION AND BIOSENSORS [9 Hours]
Physical and chemical techniques for enzyme immobilization – Adsorption, matrix entrapment, encapsulation, cross-linking, covalent binding and suitable examples – Advantages and disadvantages – Design of enzyme electrodes and their application as biosensors in industry, healthcare and environment.
[TOTAL : 45 PERIODS]
OUTCOMES:
- The knowledge on enzyme and enzyme reactions will be the key step in to proceed towards various concepts in biotechnology.
- The theoretical and practical aspects of kinetics will provide the importance and utility of enzyme kinetics towards research.
- The process of immobilization has been increased steadily in food, pharmaceutical and chemical industries and thus this study will provide simple and easy method of implementation.
- Ideas on Processing, Production and Purification of enzymes at an industrial scale will be helpful to work technologically.
TEXT BOOKS:
- Pandey A., Webb C., Soccol C. R. and Larroche C., Eds “ Enzyme Technology”,
Springer, 2006. - Buchholz, K., Kasche, V. and Bornscheuer, U., “Biocatalysts and Enzyme Technology”, WILEY–VCH, 2005.
REFERENCES:
- Drauz K., Gröger, H. and May O., “Enzyme Catalysis in Organic Synthesis: A
Comprehensive Handbook”, Volume 1, Wiley-VCH Verlag & Co, 2012. - Blanch, H.W., Clark, D.S. Biochemical Engineering, Marcel Dekker, 1997
- Bailey J.E. & Ollis, D.F. Biochemical Engineering Fundamentals, 2nd Ed., McGraw Hill, 1986
- Wiseman, Alan. Hand book of Enzyme Biotechnology, 3rd ed., Ellis Harwood 1995.
For all other B.Tech BT 4th sem syllabus go to Anna University B.Tech BIOTECHNOLOGY 4th Sem Course Structure for (R13) Batch. All details and yearly new syllabus will be updated here time to time. Subscribe, like us on facebook and follow us on google plus for all updates.
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