Anna University B.Tech BT (R13) 4th Sem Analytical Methods and Instrumentation Detailed Syllabus

Analytical Methods and Instrumentation Syllabus for B.Tech Fourth sem is covered here. This gives the details about credits, number of hours and other details along with reference books for the course.

The detailed syllabus for Analytical Methods and Instrumentation B.Tech 2016-2017 (R13) fourthsem is as follows

OBJECTIVES: To enable the students

• To have a fundamental knowledge about the Light spectrum, Absoprtion, Fluorescence NMR, Mass spectroscopy
• To acquire knowledge on the different chromotographic methods for separation of biological products.

UNIT I : INTRODUCTION TO SPECTROMETRY      [9 Hours]
Properties of electromagnetic radiation- wave properties – components of optical
instruments – Sources of radiation – wavelength selectors – sample containers – radiation transducers – Signal process and read outs – signal to noise ratio – sources of noise – Enhancement of signal to noise – types of optical instruments – Principle of Fourier Transform optical Measurements.

UNIT II : MOLECULAR SPECTROSCOPY      [9 Hours]
Molecular absorption spectrometry – Measurement of Transmittance and Absorbance – Beer’s law – Instrumentation – Applications -Theory of fluorescence and Phosphorescence – Instrumentation – Applications – Theory of Infrared absorption spectrometry – IR instrumentation – Applications – Theory of Raman spectroscopy – Instrumentation – applications.

UNIT III : MAGNETIC RESONANCE SPECTROSCOPY AND MASS SPECTROMETRY  [9 Hours]
Theory of NMR – environmental effects on NMR spectra – chemical shift- NMRspectrometers – applicatons of 1H and 13C NMR- Molecular mass spectra – ion sources – Mass spectrometer. Applications of molecular mass – Electron paramagnetic resonance- g values – instrumentation.

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[TOTAL : 45 PERIODS]

OUTCOME:
On completion of the course, students will have a better understanding of spectroscopy and the separation techniques used for biological products.

TEXT BOOKS:

• Skoog, D.A. F. James Holler, and Stanky, R.Crouch “Instrumental Methods of Analysis”. Cengage Learning , 2007.
• Willard, Hobart, etal., “Instrumental Methods of Analysis”. 7th Edition, CBS,
  1986.
• Braun, Robert D. “ Introduction to Instrumental Analysis”. Pharma Book Syndicate, 1987.
• Ewing,G.W. “Instrumental Methods of Chemical Analysis”, 5th Edition, McGrawHill, 1985

REFERENCES:

• Sharma, B.K. “Instrumental Methods of Chemical Analysis : Analytical Chemistry” Goel Publishing House, 1972.
• Haven, Mary C., etal., “Laboratory Instrumentation “. 4th Edition, John Wiley, 1995.

For all other B.Tech BT 4th sem syllabus go to Anna University B.Tech BIOTECHNOLOGY 4th Sem Course Structure for (R13) Batch. All details and yearly new syllabus will be updated here time to time. Subscribe, like us on facebook and follow us on google plus for all updates.

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