Analytical Methods and Instrumentation detail syllabus for Biotechnology (Biotech), 2017 regulation is taken from Anna University official website and presented for students of Anna University. The details of the course are: course code (BT8502), Category (PC), Contact Periods/week (3), Teaching hours/week (3), Practical Hours/week (0). The total course credits are given in combined syllabus.
For all other biotech 5th sem syllabus for b.tech 2017 regulation anna univ you can visit Biotech 5th Sem syllabus for B.Tech 2017 regulation Anna Univ Subjects. The detail syllabus for analytical methods and instrumentation is as follows.”
Course Objective:
To enable the students
- To have a fundamental knowledge about the Light spectrum, Absoprtion, Fluorescence, NMR, Mass spectroscopy
- To acquire knowledge on the different chromotographic methods for separation of biological products.
Unit I
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Unit II
Molecular Spectroscopy
Molecular absorption spectrometry – Measurement of Transmittance and Absorbance – Beers law
– Instrumentation – Applications -Theory of fluorescence and Phosphorescence – Instrumentation
– Applications – Theory of Infrared absorption spectrometry – IR instrumentation – Applications -Theory of Raman spectroscopy – Instrumentation – applications.
Unit III
Magnetic Resonance Spectroscopy and Mass Spectrometry
Theory of NMR – environmental effects on NMR spectra – chemical shift- NMR-spectrometers -applications of 1H and 13C NMR- Molecular mass spectra – ion sources – Mass spectrometer. Applications of molecular mass – Electron paramagnetic resonance- g values -instrumentation.
Unit IV
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Unit V
Electro Analysis and Surface Microscopy
Electrochemical cells- Electrode potential cell potentials – potentiometry- reference electrode – ion selective and molecular selective electrodes – Instrument for potentiometric studies – Voltametry -Cyclic and pulse voltametry- Applications of voltametry . Study of surfaces – Scanning probe microscopes – AFM and STM.
Course Outcome:
- On completion of the course, students will have a better understanding of spectroscopy and the separation techniques used for biological products.
Text Books:
- Skoog, D.A. F. James Holler, and Stanky, R.Crouch Instrumental Methods of Analysis. Cengage Learning , 2007.
- Willard, Hobart, etal., Instrumental Methods of Analysis. VIIth Edition, CBS, 1986.
- Braun, Robert D. Introduction to Instrumental Analysis. Pharma Book Syndicate, 1987.
- Ewing,G.W. Instrumental Methods of Chemical Analysis, Vth Edition, McGraw-Hill, 1985
References:
- Sharma, B.K. Instrumental Methods of Chemical Analysis: Analytical Chemistry Goel Publishing House, 1972.
- Haven, Mary C., etal., Laboratory Instrumentation . IVth Edition, John Wiley, 1995.
For detail syllabus of all other subjects of B.Tech Biotech, 2017 regulation do visit Biotech 5th Sem syllabus for 2017 Regulation.
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