Molecular Diagnostics BT 7th Sem Syllabus for VTU BE 2017 Scheme (Professional Elective-4)

Molecular Diagnostics detail syllabus for Biotechnology (BT), 2017 scheme is taken from VTU official website and presented for VTU students. The course code (17BT753), and for exam duration, Teaching Hr/week, Practical Hr/week, Total Marks, internal marks, theory marks, duration and credits do visit complete sem subjects post given below.

For all other bt 7th sem syllabus for be 2017 scheme vtu you can visit BT 7th Sem syllabus for BE 2017 Scheme VTU Subjects. For all other Professional Elective-4 subjects do refer to Professional Elective-4. The detail syllabus for molecular diagnostics is as follows.

Course Objectives:

The students acquire the

• Basic knowledge of symptoms, diagnosis, & treatment of various diseases, their importance and applications.
• Knowledge on DNA & PCR-based diagnostic techniques like FISH, SKY, CGH, PAGE, Southern Blotting, PCR-SSCP & G- Banding.
• Knowledge on Biochemical and Cell based diagnostic techniques in detail.
• Identify the different Immunodiagnostic tools available for the diagnosis of various infectious, respiratory, viral, bacterial, enteric, parasitic &mycobacterial diseases.
• Knowledge about the basic working principle, procedure & applications of various Imaging diagnostic methods such as ECG, EEG, US, CT, MRI, Endoscopy, Radiography, Nuclear Medicine, SPECT& PET.
• Knowledge on the various product development, assay development, evaluation, validation, reagent formulation & concepts and applications of biosensors for personal diabetes management.

Module 1

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Module 2

BIOCHEMICAL & CELL BASED DIAGNOSTICS : Introduction to inborn errors of metabolism, haemoglobinopathies, mucopolysaccharidoses, lipidoses, lipid profiles, HDL, LDL, Glycogen storage disorders, amyloidosis.Antibody markers, CD Markers, FACS, HLA typing, Bioassays.

Module 3

IMMUNODIAGNOSTICS: Introduction, Antigen-Antibody Reactions, Conjugation Techniques, Antibody Production, Enzymes and Signal Amplification Systems, Separation and Solid-Phase Systems, Case studies
related to bacterial, viral and parasitic infections. Diagnosis of infectious diseases, respiratory diseases (influenza, etc.) Viral diseases-HIV etc., bacterial diseases, enteric diseases, parasitic diseases and mycobacterium diseases. Phage display, immunoarrays, FACs

Module 4

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Module 5

PRODUCT DEVELOPMENT & BIOSENSORS:
Immunoassay Classification and Commercial Technologies, Assay Development, Evaluation, and Validation, Reagent Formulations and Shelf Life Evaluation, Data Analysis, Documentation, Registration, and Diagnostics Start-Ups.Concepts and applications, Biosensors for personal diabetes management, Noninvasive Biosensors in Clinical Analysis, Introduction to Biochips and their application in Health.

Course Outcomes:

After studying this course, students will be able to:

• Outline the basic concepts of health diagnostics with special emphasis on the role & importance DNA-based and PCR-based diagnostic methods.
• Describe the diagnosis of disorders such as haemoglobinopathies, mucoploysaccharidoses, lipidoses, amyloidoses using biochemical & cell-based assays.
• Identify the different immunodiagnostics & imaging diagnostic techniques.
• Explain the different ways of product & assay development methods with special emphasis on biosensors for personal diabetes management.

Reference Books:

1. Molecular Biotechnology
2. Genetic Engineering
3. Recombinant DNA
4. Vectors

Text Books:

1. Medical Informatics, a Primer by Mohan Bansal, TMH publications.
2. Tietz Textbook of Clinical Chemistry
3. Commercial Biosensors
4. Essentials of Diagnostic Microbiology
5. Diagnostic Microbiology
6. Molecular Biotechnology- Principles and Applications of recombinant DNA
7. Principles of gene manipulation- An introduction to genetic engineeringGenes VIII

For detail syllabus of all other subjects of BE Bt, 2017 regulation do visit Biotech 7th Sem syllabus for 2017 Regulation.

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